Preliminary application of micro-SPECT/CT imaging by 99mTc-tricine-EDDA-HYNIC-c-Met for non-small-cell lung cancer
Preliminary application of micro-SPECT/CT imaging by 99mTc-tricine-EDDA-HYNIC-c-Met for non-small-cell lung cancer
复制标题
99m Tc-tricine-EDDA-HYNIC-c-Met显微SPECT/CT成像在非小细胞肺癌中的初步应用
DOI：
10.1111/cbdd.13432
复制
发表时间：
2019-04-01
影响因子：
3
通讯作者：
Shi，Hongcheng
中科院分区：
文献类型：
--
作者：
Zhang，Yiqiu;Meng，Lanfang;Shi，Hongcheng
ObjectiveA novel99mTc‐tricine‐EDDA‐Hynic‐c‐Met molecular probe was synthetized, and nude mice models of human non‐small‐cell lung cancer (NSCLC) were established in order to preliminarily investigate that whether the molecular probe can be used to screen c‐Met inhibitor for targeted drug therapy in NSCLC.MethodsWith Hynic as the chelating agent,99mTcO4‐labeled c‐Met receptor was used to synthetize99mTc‐tricine‐EDDA‐HYNIC‐c‐Met. Two nude mice successfully transplanted with H1993 tumor and two nude mice transplanted with H292 tumor were injected with99mTc‐tricine‐EDDA‐HYNIC‐c‐Met through the tail vein. After 2 and 4 hr, micro‐SPECT/CT imaging was performed.Resultsmicro‐SPECT/CT imaging of nude mice transplanted with H1993 and H292 tumors using99mTc‐tricine‐EDDA‐HYNIC‐c‐Met showed that uptake of99mTc‐tricine‐EDDA‐HYNIC‐c‐Met was high in H1993 tumor, while it was mild in H292 tumor both at 2 and 4 hr postinjection. Semiquantitative analysis of the ratio of radioactivity intensity at tumor site to radioactivity intensity of adjacent muscles (T/N value) showed that the average T/N value of H1993 and H292 tumors at 2 hr was 3.90 and 2.85, while it was 4.88 and 2.36 at 4 hr.Conclusionmicro‐SPECT/CT imaging through99mTc‐tricine‐EDDA‐HYNIC‐c‐Met can be used to screen c‐Met indicators of NSCLC in H1993 nude mice models for targeted drug therapy.
