Magnetometric evaluation for the effects of silicon carbide whiskers on alveolar macrophages.
Magnetometric evaluation for the effects of silicon carbide whiskers on alveolar macrophages.
复制标题
磁力评估碳化硅晶须对肺泡巨噬细胞的影响。
作者：
Mitsuyasu Watanabe;M. Okada;Y. Aizawa;Yasuhiro Sakai;Shohei Yamashina;Makoto Kotani
肺泡巨噬细胞被认为在各种类型的粉尘暴露引起的肺纤维化中起重要作用。在本实验中，我们主要通过独特的磁测量法以及已建立的方法如乳酸脱氢酶（LDH）活性、凋亡测量和形态学观察来评估碳化硅晶须（SiC）对肺泡巨噬细胞的影响。从叙利亚金黄地鼠支气管肺泡灌洗获得的肺泡巨噬细胞在体外暴露于Fe_3O_4 18小时作为磁测量的指示剂和SiC用于实验。在巨噬细胞单独吞噬Fe_3O_4的外部磁场停止后，观察到reflux磁场的快速降低，即所谓的“弛豫”，而同时暴露于SiC的巨噬细胞的弛豫延迟。从SiC暴露的巨噬细胞释放到培养基中的LDH并不显着高于对照组，但它增加剂量依赖性。通过DNA梯状条带检测和形态学观察，发现60 μ g/ml SiC可诱导巨噬细胞凋亡。电子显微镜检查显示，在暴露于SiC的巨噬细胞的核和细胞器的不规则的形式。磁性测定、LDH释放和电镜观察表明SiC对肺泡巨噬细胞有轻度的细胞毒性。
Alveolar macrophages are thought to play an important role in fibrogenesis in the lungs caused by various types of exposure to dust. In this experiment, we evaluated the effect of silicon carbide whiskers (SiC) on alveolar macrophages mainly by unique magnetometry and also by established methods such as lactate dehydrogenase (LDH) activity, apoptosis measurement and morphological observations. Alveolar macrophages obtained from Syrian golden hamsters by bronchoalveolar lavages were exposed in vitro to Fe3O4 for 18 hours as an indicator for magnetometry and SiC for experiments. A rapid decrease of the remanent magnetic field, so called "relaxation", was observed after cessation of an external magnetic field in macrophages phagocytizing Fe3O4 alone, while relaxation was delayed in those concurrently exposed to SiC. Release of LDH from SiC-exposed macrophages into the medium was not significantly higher than the controls, but it increased dose-dependently. Apoptosis was recognized in macrophages exposed to 60 micrograms/ml of SiC by the DNA ladder detection method and morphological observations. Electron microscopic examination revealed irregular forms of nuclei and organellas in macrophages exposed to SiC. Magnetometry, LDH release and electron microscopic observation indicated mild cytotoxicity of SiC to alveolar macrophages.
内源性一氧化氮对体外和体内大鼠肝细胞线粒体呼吸的影响。
DOI：
10.1001/archsurg.1991.01410260074010
发表时间：
1991
期刊：
Archives of surgery (Chicago, Ill. : 1960)
影响因子：
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作者：
Stadler,J;Curran,RD;Ochoa,JB;Harbrecht,BG;Hoffman,RA;Simmons,RL;Billiar,TR
通讯作者：
Billiar,TR