LIPOPOLYSACCHARIDE OF PSEUDOMONAS AERUGINOSA WITH SPECIAL REFERENCE TO PYOCIN R RECEPTOR ACTIVITY
LIPOPOLYSACCHARIDE OF PSEUDOMONAS AERUGINOSA WITH SPECIAL REFERENCE TO PYOCIN R RECEPTOR ACTIVITY
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铜绿假单胞菌的脂多糖，特别涉及脓毒素 R 受体活性
DOI：
10.2323/jgam.19.115
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发表时间：
1973
影响因子：
1.2
通讯作者：
F. Egami
中科院分区：
文献类型：
--
作者：
K. Ikeda;F. Egami
1)采用苯酚法从铜绿假单胞菌P14中分离出具有pyocin R受体活性的脂多糖。在脱氧胆酸钠存在下热处理后，通过Sephadex G-100凝胶过滤，脂多糖被解离并分级为富含氨基糖的级分和脂多糖亚基。 2)在脱氧胆酸钠存在下，脂多糖亚基(分子量12, 000-16, 000)没有受体活性，但在不存在脱氧胆酸钠时它们重新结合，活性为恢复了。因此，亚基可被视为受体活性的化学实体。3)对经酸处理消除“脂质A”后重新结合的脂多糖和碳水化合物片段(分子量1, 300-1, 500)进行化学分析。前者含有葡萄糖、鼠李糖、庚糖、葡萄糖胺、半乳糖胺、奎诺糖胺、岩藻糖胺和2-酮-3-脱氧糖酸。后者仅含有葡萄糖、鼠李糖、庚糖、半乳糖胺、2-酮-3-脱氧糖酸和磷酸盐。
1) Lipopolysaccharide with pyocin R receptor activity was isolated from Pseudomonas aeruginosa P14 by the phenol method. Lipopolysaccharide was dissociated and fractionated into amino-sugar-rich fraction and lipopolysaccharide subunits by Sephadex G-100 gel filtration after heat treatment in the presence of sodium deoxycholate.2) The lipopolysaccharide subunits (mol. wt. 12, 000-16, 000) had no receptor activity in the presence of sodium deoxycholate, but they were reassociated in the absence of sodium deoxycholate and the activity was recovered. Therefore, the subunits may be regarded as the chemical entity of the receptor activity.3) Chemical analysis of the reassociated lipopolysaccharide and the carbohydrate fragment (mol. wt. 1, 300-1, 500) after acid treatment for the elimination of "lipid A" was carried out. The former contained glucose, rhamnose, heptose, glucosamine, galactosamine, quinovosamine, fucosamine, and a 2-keto-3-deoxy-sugar acid. The latter contained only glucose, rhamnose, heptose, galactosamine, 2-keto-3-deoxy-sugar acid, and phosphate.