Dynamic pH junction technique for on-line preconcentration of peptides in capillary electrophoresis
Dynamic pH junction technique for on-line preconcentration of peptides in capillary electrophoresis
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DOI：
10.1016/j.chroma.2005.03.069
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发表时间：
2005-06-24
影响因子：
4.1
通讯作者：
Terabe, S
中科院分区：
文献类型：
--
作者：
Monton, MRN;Imami, K;Terabe, S
A method based on the presence of a dynamic pH junction within the capillary to induce band narrowing for enhanced detection sensitivity for some peptides is presented. This technique is predicated on a sharp reduction in an analyte's migration velocity following a reversal of its electrophoretic direction from the acidic sample zone to the basic BGS zone. Larger-than-usual injection volumes of samples in relatively high-conductivity matrices were enabled, without degrading peak shape, resolution and efficiency. The size of the original sample plug was reduced by as much as 38-fold, and improvement in detector response in terms of peak height by as much as 124-fold was obtained. The effects of pH and concentration of the sample matrix, and the length of sample injection on the efficiency of the technique are discussed. (c) 2005 Elsevier B.V All rights reserved.
