Effects of R gene-mediated resistance in Brassica napus (oilseed rape) on asexual and sexual sporulation of Pyrenopeziza brassicae (light leaf spot)
Effects of R gene-mediated resistance in Brassica napus (oilseed rape) on asexual and sexual sporulation of Pyrenopeziza brassicae (light leaf spot)
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DOI：
10.1111/j.1365-3059.2011.02529.x
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发表时间：
2012-06-01
期刊：
影响因子：
2.7
通讯作者：
Fitt, B. D. L.
中科院分区：
文献类型：
--
作者：
Boys, E. F.;Roques, S. E.;Fitt, B. D. L.
油菜（Brassica napus） R基因介导的抗性表型研究。研究了伊莫拉对植物轻叶斑病病原菌（Pyrenopeziza brassicae）的抗性。使用人口翻了一番单倍体B.similar显著映射为反抗种族隔离P.similar brassicae,发展视觉症状和无症状的增长特征之后使用定量PCR和扫描电子显微镜对叶子的抗/敏感线P.similar悬浮液接种brassicae分生孢子。最初，在受控环境实验中，与芸苔属植物相似的紫苔属植物的生长不受影响；接种后8 d，绿活组织中部分表皮细胞塌陷，出现黑色斑点。在13 ~ 36 dpi期间，与芸苔科DNA相似的p .数量没有增加，也没有出现无性孢子形成（针囊/脓疱）。相比之下，在此期间，与芸苔科DNA相似的p .增加了300倍，并且在感病cv的叶片中大量产生无性孢子。顶点。然而，当叶片组织衰老时，与芸苔科类似的p . DNA在抗性品种中迅速增加，而在敏感品种中则没有增加，并且在衰老组织中有性孢子（apothecia）丰富。这些结果与对照条件和田间试验的观察结果是一致的，这些试验来自于为这种抗性而分离的作图群体。控制环境和田间试验结果分析表明，这种抗性是由位于A1染色体上的单个R基因介导的。
The phenotype of the R gene-mediated resistance derived from oilseed rape (Brassica napus) cv. Imola against the light leaf spot plant pathogen, Pyrenopeziza brassicae, was characterized. Using a doubled haploid B.similar to napus mapping population that segregated for resistance against P.similar to brassicae, development of visual symptoms was characterized and symptomless growth was followed using quantitative PCR and scanning electron microscopy on leaves of resistant/susceptible lines inoculated with suspensions of P.similar to brassicae conidia. Initially, in controlled-environment experiments, growth of P.similar to brassicae was unaffected; then from 8 days post-inoculation (dpi) some epidermal cells collapsed (black flecking) in green living tissue of cv. Imola and from 13 to 36 dpi there was no increase in the amount of P.similar to brassicae DNA and no asexual sporulation (acervuli/pustules). By contrast, during this period there was a 300-fold increase in P.similar to brassicae DNA and extensive asexual sporulation in leaves of the susceptible cv. Apex. However, when leaf tissue senesced, the amount of P.similar to brassicae DNA increased rapidly in the resistant but not in the susceptible cultivar and sexual sporulation (apothecia) was abundant on senescent tissues of both. These results were consistent with observations from both controlled condition and field experiments with lines from the mapping population that segregated for this resistance. Analysis of results of both controlled-environment and field experiments suggested that the resistance was mediated by a single R gene located on chromosome A1.