Central and peripheral administration of human relaxin-2 to adult male rats inhibits food intake.
Central and peripheral administration of human relaxin-2 to adult male rats inhibits food intake.
复制标题
对成年雄性大鼠中枢和外周施用人松弛素-2 会抑制食物摄入。
DOI：
10.1111/j.1463-1326.2010.01302.x
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
McGowan BM
中科院分区：
文献类型：
--
作者：
McGowan BM
目的：松弛素是一种与妊娠和哺乳有关的多肽激素。它主要由黄体和胎盘分泌，但在许多其他组织中表达，包括心脏和大脑。在脑内，松弛素在嗅觉和边缘系统、皮质和下丘脑弓状核（ARC）中表达。其同源受体松弛素家族肽受体1（RXFP 1）也广泛表达于大脑中，包括下丘脑ARC和室旁核（PVN），这些区域在食欲调节中很重要。本研究的目的是调查松弛素是否通过中枢下丘脑circuits.Methods影响食物摄入量：人类形式的松弛素，人类松弛素-2（H2）的中央和外周给予雄性Wistar大鼠和食物摄入量的测量。结果：脑室内（ICV）给予H2显著降低了黑暗早期1小时的摄食量[2.95 ± 0.45 g（生理盐水）vs. 0.95 ± 0.18 g（180 pmol H2），p < 0.001]。ICV管理的H2减少了摄食行为，增加了梳理和头朝下的行为。脑室旁注射H2显著降低了黑暗早期1小时的摄食量[3.13 ± 0.35 g（生理盐水）vs. 1.35 ± 0.33 g（18 pmol H2），p < 0.01，1.61 ± 0.31 g（180 pmol H2），p < 0.05和1.23 ± 0.32 g（540 pmol H2），p < 0.001]。腹膜内（IP）给予H2显著降低了早期黑暗期的1小时摄食量[4.63 ± 0.46 g（溶剂）vs. 3.08 ± 0.15 g（66 nmol H_2），p < 0.01，3.00 ± 0.17 g（200 nmol H_2），p < 0.01和2.26 ± 0.36 g（660 nmol H_2），p < 0.001]。这种效应可以通过PVN和/或其他脑区域介导。
Aim:Relaxin is a polypeptide hormone involved in pregnancy and lactation. It is mainly secreted by the corpus luteum and placenta, but is expressed in a number of other tissues, including heart and brain. Within the brain, relaxin is expressed in the olfactory and limbic systems, the cortex and the hypothalamic arcuate nucleus (ARC). Its cognate receptor, relaxin family peptide receptor 1 (RXFP1), is also widely expressed in the brain, including the hypothalamic ARC and paraventricular nucleus (PVN), areas important in appetite regulation. The aim of this study was to investigate whether relaxin influences food intake through central hypothalamic circuits.Methods:The human form of relaxin, human relaxin‐2 (H2) was administered centrally and peripherally to male Wistar rats and food intake measured. Behaviour was also assessed.Results:Intracerebroventricular (ICV) administration of H2 significantly decreased 1‐h food intake in the early dark phase [2.95 ± 0.45 g (saline) vs. 0.95 ± 0.18 g (180 pmol H2), p < 0.001]. ICV administration of H2 decreased feeding behaviour and increased grooming and headdown behaviour. Intraparaventricular injections of H2 significantly decreased 1‐h food intake in the early dark phase [3.13 ± 0.35 g (saline) vs. 1.35 ± 0.33 g (18 pmol H2), p < 0.01, 1.61 ± 0.31 g (180 pmol H2), p < 0.05 and 1.23 ± 0.32 g (540 pmol H2), p < 0.001]. Intraperitoneal (IP) administration of H2 significantly decreased 1‐h food intake in the early dark phase [4.63 ± 0.46 g (vehicle) vs. 3.08 ± 0.15 g (66 nmol H2), p < 0.01, 3.00 ± 0.17 g (200 nmol H2), p < 0.01 and 2.26 ± 0.36 g (660 nmol H2), p < 0.001].Conclusions:Central and peripheral administration of H2 reduces the food intake in rats. This effect may be mediated via the PVN and/or other brain regions.