STRUCTURE OF TOBACCO MOSAIC VIRUS
STRUCTURE OF TOBACCO MOSAIC VIRUS
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DOI：
10.1038/175379a0
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发表时间：
1955-01-01
期刊：
影响因子：
64.8
通讯作者：
FRANKLIN, RE
中科院分区：
文献类型：
--
作者：
FRANKLIN, RE
X射线衍射研究表明，烟草花叶病毒的蛋白质是以分子量约为29，000的结构等效单元的形式排列在螺距为23的螺旋上，在螺旋的3个转角上有3n + 1个这样的单元，其中n可能等于12。这些单位似乎被细分，沿着一个平面近似垂直于螺旋轴，分为2个等效或接近等效的子单位。这些亚基的分子量与通过化学方法获得的最小分子量一致。在螺旋的一圈和下一圈之间有一个重要的结构不连续性，这似乎与颗粒的外部凹槽有关。这种表面特征使病毒具有相当大的外表面，这也许可以解释烟草花叶病毒化学修饰的惊人多样性和程度，而不破坏颗粒，在某些情况下，不破坏其感染性。
X-ray diffraction studies indicate that the protein of tobacco mosaic virus is in the form of structurally equivalent units of molecular weight about 29,000 arranged on a helix of pitch 23 A, there being 3n + 1 such units on 3 turns of the helix, with n probably equal to 12. These units appear to be subdivided, along a plane approximately perpendicular to the axis of the helix, into 2 equivalent or near-equivalent sub-units. The molecular weight of these sub-units is consistent with the minimum molecular weight obtained by chemical methods. There is an important structural discontinuity between one turn of the helix and the next, and this appears to be associated with an external grooving of the particle. This feature of the surface, which gives the virus a rather large external surface, may perhaps account for the surprising variety and extent of chemical modifications which it is possible to make in tobacco mosaic virus without breaking up the particle and, in some cases, without destroying its infectivity.