Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer
Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer
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人结肠癌中胸腺素-α mRNA 表达与 c-myc 相关
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
Liwei Chen
中科院分区：
文献类型：
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作者：
M. Mori;G. Barnard;R. Staniunas;J. Jessup;G. Steele;Liwei Chen
胸腺原蛋白（pt - α）是一种参与细胞增殖的核蛋白。据报道，pt - α的转录在体外受c-myc基因的调控。我们在人类结肠癌减去正常粘膜减去cDNA文库中鉴定出pt - α过表达。Northern blot（信使RNA）分析显示，与邻近正常组织相比，人类结直肠癌中pt - α和c-myc基因均过表达。免疫组织化学对pt - α和c-myc的研究支持了这些发现。pt - α、c-myc信使RNA表达与大肠癌Dukes分期无相关性；或者两者之间的关系以及肌动蛋白信使RNA的表达。然而，pt - α表达与c-myc表达之间存在显著相关性（P < 0.001）。这些发现支持了人类结直肠癌中pt - α基因转录可能与c-myc基因相关并可能受其控制的假设。
Prothymosin alpha (PT-alpha) is a nuclear protein involved in cell proliferation. Transcription of PT-alpha has been reported to be regulated by the c-myc gene in vitro. We identified PT-alpha as being overexpressed in a human colon cancer minus normal mucosa subtraction cDNA library. Northern blot (messenger RNA) analysis showed that both PT-alpha and c-myc genes were overexpressed in human colorectal cancers compared with adjacent normal tissues. Immunohistochemical studies for PT-alpha and c-myc supported these findings. There was no correlation between PT-alpha or c-myc messenger RNA expression and Dukes' stage of colorectal cancer; or between either of these two and actin messenger RNA expression. There was, however, a significant correlation between the PT-alpha expression and c-myc expression (P < 0.001). These findings support the hypothesis that PT-alpha gene transcription may be associated with, and possibly under the control of, the c-myc gene in human colorectal cancers.