In vitro evaluation of combined sulfated silk fibroin scaffolds for vascular cell growth.
In vitro evaluation of combined sulfated silk fibroin scaffolds for vascular cell growth.
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DOI：
10.1002/mabi.201200470
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发表时间：
2013-06
影响因子：
4.6
通讯作者：
Haifeng Liu;Xili Ding;Yanxue Bi;Xianghui Gong;Xiaoming Li;Gang Zhou;Yubo Fan
中科院分区：
文献类型：
--
作者：
Haifeng Liu;Xili Ding;Yanxue Bi;Xianghui Gong;Xiaoming Li;Gang Zhou;Yubo Fan
通过用硫酸化丝素海绵对针织丝素支架进行改性，制备了组合硫酸化丝素支架。体外血液相容性评价表明，复合硫酸化丝素蛋白支架可降低血小板的粘附和活化，延长活化部分凝血活酶时间（APTT）、凝血酶时间（TT）和凝血酶原时间（PT）。研究了猪血管内皮细胞（EC）和平滑肌细胞（SMCs）在支架上的反应，以评价支架的细胞相容性。将血管细胞接种在支架上并培养2周。支架显示增强的EC粘附、增殖和细胞功能的维持。此外，支架抑制SMC增殖并诱导收缩SMC标记基因的表达。
A combined sulfated silk fibroin scaffold is fabricated by modifying a knitted silk scaffold with sulfated silk fibroin sponges. In vitro hemocompatibility evaluation reveals that the combined sulfated silk fibroin scaffolds reduce platelet adhesion and activation, and prolong the activated partial thromboplastin time (APTT), thrombin time (TT), and prothrombin time (PT). The response of porcine endothelial cells (ECs) and smooth muscle cells (SMCs) on the scaffolds is studied to evaluate the cytocompatibility of the scaffolds. Vascular cells are seeded on the scaffolds and cultured for 2 weeks. The scaffolds demonstrate enhanced EC adhesion, proliferation, and maintenance of cellular functions. Moreover, the scaffolds inhibit SMC proliferation and induce expression of contractile SMC marker genes.