Rhesus rhadinovirus establishes a latent infection in B lymphocytes in vivo
Rhesus rhadinovirus establishes a latent infection in B lymphocytes in vivo
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DOI：
10.1128/jvi.73.9.7874-7876.1999
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发表时间：
1999-09-01
影响因子：
5.4
通讯作者：
Wong, SW
中科院分区：
文献类型：
--
作者：
Bergquam, EP;Avery, N;Wong, SW
最近的DNA序列分析表明，恒河猴Rhadinovirus（RRV）是亲淋巴细胞γ-2疱疹病毒家族的成员。为了确定RRV是否是嗜淋巴细胞的，通过免疫磁珠去除法分离自然感染猴的外周血单核细胞，并通过病毒分离和巢式PCR分析RRV的存在。在CD 20（+）富集组分中回收和一致检测RRV清楚地表明B淋巴细胞是病毒持续存在的主要部位。
Recent DNA sequence analysis indicates that rhesus rhadinovirus (RRV) is a member of the lymphotropic gamma-2 herpesvirus family. To determine if RRV is lymphotropic, peripheral blood mononuclear cells from naturally infected monkeys were separated by immunomagnetic bead depletion and analyzed for the presence of RRV by virus isolation and nested PCR The recovery and consistent detection of RRV in the CD20(+)-enriched fraction clearly demonstrates that B lymphocytes are a major site of virus persistence.