The induction of polyploidy in Nicotiana species and species hybrids
The induction of polyploidy in Nicotiana species and species hybrids
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DOI：
10.1093/oxfordjournals.jhered.a104748
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发表时间：
1939-07-01
影响因子：
3.1
通讯作者：
Smith, HH
中科院分区：
文献类型：
--
作者：
Smith, HH
用0.4%秋水仙素处理烟草的分生组织，用0.2%、0.4%和0.8%秋水仙素处理种子，获得了染色体数目加倍的烟草成熟植株。烟草N. rustica、黄绿拟单胞菌N. glauca，F1（N.烟草X N. glauca），F1（N. tabacum X N. sylvestris），F1（N.黄毛苔属glauca）和部分N.野蔷薇X N.烟草分离株同源四倍体N.烟草N. rustica和N.与二倍体相比，glauca的特征在于较小的植株习性、较小且较厚的叶片和较大的气孔，以及增加的花粉不育性。对N.烟草N. rustica的种子比二倍体大。异源四倍体的不育F_1（N. tabacum X N. glauca）和（N.烟草sylvestris）可育，叶片宽而厚，气孔大，生长习性与F1相似。矮秆杂种的异源四倍体N.黄毛苔属glauca的植株活力、叶片大小和花粉育性均比F1有显著提高。超多倍体N.野蔷薇X N.与未加倍的姊妹株相比，tabacum分离株具有较小的植株习性、较小和较厚的叶片以及较大的气孔，并且花粉育性增加。
By meristem treatments with 0.4% colchicine and seed treatments with 02, 0.4, and 0.8% colchicine mature plants with a double number of chromosomes were obtained of the following Nicotiana species and hybrids: N. tabacum, N. rustica, N. glauca, F1 (N. tabacuto X. N. glauca), F1 (N. tabacum X N. sylvestris), F1 (N. glutinosa X N. glauca), and some N. rustica X N. tabacum segregants. The autotetraploids of N. tabacum, N. rustica and N. glauca were characterized by smaller plant habit, smaller and thicker leaves with larger stomata and increased pollen sterility compared to the diploids. The polyploids of N. tabacum and N. rustica had larger seed than the diploids. Allotetraploids of the sterile F1''s (N. tabacum X N. glauca) and (N. tabacum XN. sylvestris) were fertile, had wider and thicker leaves with larger stomata and were similar in growth habit to the F1''s. Allotetraploids of the dwarf hybrid, N. glutinosa X N. glauca, showed a marked increase in plant vigor, leaf size and pollen fertility over the F1. Hyperpolyploids of N. rustica X N. tabacum segregants had smaller plant habit, smaller and thicker leaves with larger stomata and increased pollen fertility compared to un-doubled sister plants.