Telomerase activity in human endometrium.
Telomerase activity in human endometrium.
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发表时间：
1997-02
期刊：
影响因子：
11.2
通讯作者：
S. Kyo;M. Takakura;T. Kohama;M. Inoue
中科院分区：
文献类型：
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作者：
S. Kyo;M. Takakura;T. Kohama;M. Inoue
人子宫内膜在月经周期中经历了增殖和分泌活动的复杂变化模式。在本研究中，端粒酶活性在正常子宫内膜检测使用非放射性同位素pcr为基础的端粒重复扩增协议测定。在检查的60个正常子宫内膜样本中检测到不同水平的端粒酶活性，这取决于月经周期的阶段。在21个增殖期子宫内膜样本中，20个（95%）表达端粒酶活性，而19个分泌期或月经期子宫内膜样本中有8个（42%）表达端粒酶活性（P = 0.002）。来自绝经后妇女萎缩性子宫内膜的9个样本中有5个（56%）也表达端粒酶活性。经100倍稀释后，21例增殖期子宫内膜样品中有11例（52%）表达了高端粒酶活性，而19例分泌期或月经期子宫内膜样品和9例绝经后子宫内膜样品均未检测到高端粒酶活性（P < 0.001）。增殖后期活性最高，随着分泌期的进行活性急剧下降。令人惊讶的是，在晚期增殖阶段检测到的端粒酶活性水平与子宫内膜癌检测到的水平相当。增殖细胞核抗原表达的免疫组化分析显示，端粒酶活性与子宫内膜细胞增殖活性密切相关。这些发现表明，正常子宫内膜表达端粒酶，其活性在月经周期中发生显著变化，这表明端粒酶是一种受调节的酶，与细胞增殖有关，激素功能可能参与其调节。
Human uterine endometrium undergoes a complex pattern of changes in proliferation and secretory activity during the menstrual cycle. In the present study, telomerase activity in normal endometrium was examined using a non-radioisotope PCR-based telomeric repeat amplification protocol assay. Various levels of telomerase activity were detected in the 60 normal endometrial samples examined, depending on the phase of the menstrual cycle. Of 21 proliferative-phase endometrial samples, 20 (95%) expressed telomerase activity, whereas 8 of 19 (42%) secretory-phase or menstrual endometrial samples did (P = 0.002). Five of nine (56%) samples from atrophic endometrium from postmenopausal women also expressed telomerase activity. Eleven of 21 (52%) endometrial samples in the proliferative phase expressed high telomerase activity detectable after 100-fold dilution of extracts, whereas none of the 19 endometrial samples from the secretory phase or during menstruation and none of the 9 postmenopausal endometrial samples did (P < 0.001). The highest activity was observed in the late proliferative phase, but activity dramatically decreased with the progression of the secretory phase. Surprisingly, the levels of telomerase activity detected in the late proliferative phase were comparable to those detected in the endometrial cancers examined. Immunohistochemical analysis of the expression of proliferating cell nuclear antigen revealed that telomerase activity is closely correlated with endometrial cell proliferative activity. These findings indicate that normal endometrium expresses telomerase, the activity of which changes dramatically over the course of the menstrual cycle, suggesting in turn that telomerase is a regulated enzyme linked to cellular proliferation and that hormone functions may be involved in its regulation.