The influence of fasting/refeeding on the lipoprotein lipase activity of adipose tissue and muscle
The influence of fasting/refeeding on the lipoprotein lipase activity of adipose tissue and muscle
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DOI：
10.1590/s0100-879x2001001100006
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发表时间：
2001-11-01
影响因子：
2.3
通讯作者：
Botion, LM
中科院分区：
文献类型：
--
作者：
Botion, LM
脂肪组织和肌肉中的脂蛋白脂肪酶活性受到食物摄入模式变化的调节。我们测量了体重 200-250 克（类似于 12 周）的雄性 Wistar 大鼠（N = 6-10）在禁食 24 小时后的重新喂养/禁食状态期间的脂肪组织和肌肉中的总脂蛋白脂肪酶活性。使用含有[H-3]-三油酸甘油酯的底物评估组织匀浆中的脂蛋白脂肪酶活性，并通过液体闪烁提取和定量释放的[H-3]游离脂肪酸。脂肪组织脂蛋白脂肪酶活性在重新喂食后 2 小时内没有完全恢复（随意喂食值的 60%）。即使在重新喂食后 2 小时，心脏脂蛋白脂肪酶活性仍保持增加（随意喂食值的 100%），而在比目鱼肌和膈肌中未观察到显着变化。脂肪组织脂蛋白脂肪酶活性始终高于骨骼肌或心脏的最高值。因此，脂肪组织，而不是肌肉，可能对三酰甘油的清除做出主要贡献。在重新喂养的最初几个小时内，脂肪组织和心肌中同时存在相对较高的脂蛋白脂肪酶活性，因此在此期间心肌可能对三酰甘油清除有显着贡献。结果表明，在禁食期间，脂蛋白脂肪酶活性的增加提供了循环三酰甘油中游离脂肪酸的补充来源，使心脏能够维持其持续的高能量消耗。
Lipoprotein lipase activity in adipose tissue and muscle is modulated by changes in the pattern of food intake. We have measured total lipoprotein lipase activity in adipose tissue and muscle of male Wistar rats (N = 6-10), weighing 200-250 g (similar to 12 weeks), during the refeeding/fasting state following 24 h of fasting. Lipoprotein lipase activity in tissue homogenates was evaluated using a [H-3]-triolein-containing substrate, and released [H-3]-free fatty acids were extracted and quantified by liquid scintillation. Adipose tissue lipoprotein lipase activity did not completely recover within 2 h of refeeding (60% of refed ad libitum values). Cardiac lipoprotein lipase activity remained increased even 2 h after refeeding (100% of refed ad libitum values), whereas no significant changes were observed in the soleus and diaphragm muscles. Adipose tissue lipoprotein lipase activities were consistently higher than the highest skeletal muscle or heart values. It is therefore likely that adipose tissue, rather than muscle makes the major contribution to triacylglycerol clearance. There was concomitant relatively high lipoprotein lipase activity in both adipose tissue and cardiac muscle during the first few hours of refeeding, therefore cardiac muscle may contribute significantly to triacylglycerol clearance during this period. The results suggest that during fasting, increased lipoprotein lipase activity provides a complementary source of free fatty acids from circulating triacylglycerol, allowing the heart to maintain its continuous, high-energy expenditure.