Chromosomal integrity of freeze-dried mouse spermatozoa after 137Cs γ-ray irradiation
Chromosomal integrity of freeze-dried mouse spermatozoa after 137Cs γ-ray irradiation
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DOI：
10.1016/j.mrfmmm.2004.08.001
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发表时间：
2004-11-22
影响因子：
2.3
通讯作者：
Kamiguchi, Y
中科院分区：
文献类型：
--
作者：
Kusakabe, H;Kamiguchi, Y
这项研究表明，冷冻干燥的小鼠精子具有很强的抵抗（CS）-C-137 γ射线照射剂量高达8戈伊。将冷冻干燥的小鼠精子再水化，并采用卵胞浆内单精子注射（ICSI）技术注射到小鼠卵母细胞中。大多数卵母细胞可以在ICSI后通过使用冷冻干燥前后的γ射线照射精子来激活。在第一次卵裂中期分析精子染色体补体。冷冻干燥前照射的精子染色体畸变呈剂量依赖性增加。然而，没有增加的卵母细胞染色体畸变时，观察到受精的精子冷冻干燥后，与冷冻干燥的精子没有被照射。这些结果表明，冷冻干燥精子的染色体完整性，以及它们激活卵母细胞的能力，受到保护，从γ射线照射的剂量，染色体损伤被发现是强烈诱导悬浮在溶液中的精子。(C)2004年爱思唯尔B. V.保留所有战斗。
This study demonstrated that freeze-dried mouse spermatozoa possess strong resistance to (CS)-C-137 gamma-ray irradiation at doses of up to 8 Gy. Freeze-dried mouse spermatozoa were rehydrated and injected into mouse oocytes with an intracytoplasmic sperm injection (ICSI) technique. Most oocytes can be activated after ICSI by using spermatozoa irradiated with gamma-rays before and after freeze-drying. Sperm chromosome complements were analyzed at the first cleavage metaphase. Chromosome aberrations increased in a dose-dependent manner in the spermatozoa irradiated before freeze-drying. However, no increase in oocytes with chromosome aberrations was observed when fertilized by spermatozoa that had been irradiated after freeze-drying, as compared with freeze-dried spermatozoa that had not been irradiated. These results suggest that both the chromosomal integrity of freeze-dried spermatozoa, as well as their ability to activate oocytes, were protected from gamma-ray irradiation at doses at which chromosomal damage is found to be strongly induced in spermatozoa suspended in solution. (C) 2004 Elsevier B.V. All fights reserved.