Bloom of tetracycline resistance genes in mudflats following fertilization is attributed to the increases in the shared potential hosts between soil and organic fertilizers
Bloom of tetracycline resistance genes in mudflats following fertilization is attributed to the increases in the shared potential hosts between soil and organic fertilizers
复制标题
施肥后泥滩中四环素抗性基因的大量繁殖归因于土壤和有机肥料之间共享潜在宿主的增加
DOI：
10.1007/s11356-021-16676-3
复制
发表时间：
2021-09-28
影响因子：
5.8
通讯作者：
Hu, Jian
中科院分区：
文献类型：
--
作者：
Kang, Yijun;Xu, Wenjie;Hu, Jian
采用田间试验方法，研究了不同施用量的污泥和蚯蚓粪对滩涂土壤中四环素抗性基因（TRG）及其潜在宿主的影响。定量PCR结果表明，无论是添加污泥或蚯蚓粪的土壤中有更丰富的TRGs相比，未施肥的土壤。这种情况在施用污泥的土壤中更为明显，特别是在高施用量下。蚯蚓堆肥在滩涂改良中具有良好的应用前景。未施肥土壤中的tI 1丰度显著高于施肥土壤和施肥土壤。intI1的潜在寄主与其它含TRG的寄主没有共同的寄主，表明intI1对TRG在滩涂的传播影响不大。此外，肥料中TRGs的专性宿主并不高于未施肥土壤中的专性宿主，说明施肥对外源TRGs引入土壤的影响不大。土壤和肥料之间的共享主机是最高的四个可能的来源，大大有助于开花的TRGs施肥后。不同的有机肥对TRG的潜在寄主有明显的影响。RDA分析进一步表明，土壤养分对共享潜在寄主的丰度有影响。这些结果表明，施肥后土壤中TRG的发展取决于土壤和肥料之间具有相似生态位的共享潜在宿主。
A field experiment was carried out in mudflats adjacent to the Yellow Sea, China, amended with sewage sludge and vermicompost by one-time input at different rates to reveal the fates of tetracycline resistance genes (TRGs) and their potential hosts in the soils. Quantitative PCR results showed that soils added with either sludge or vermicompost had more abundant TRGs compared with the non-fertilized soil. This situation was more obvious in sludge fertilized soils especially at high application rates. Vermicompost exhibited a promising outlook for improvement of the mudflats. The abundances ofintI1 in the non-fertilized soils were significantly higher than those in fertilizers and fertilized soils. The potential hosts forintI1 were not shared with other TRGs-contained hosts, indicating thatintI1 had little effects on the dissemination of TRGs in the mudflats. Moreover, the exclusive hosts for TRGs in fertilizers were not higher than those in the non-fertilized soils, illustrating little effects of fertilization on the introduction of exogenous TRGs into soil. The shared hosts between soil and fertilizers were highest among four possible sources, contributing vastly to the bloom of TRGs following fertilization. It was also shown that different organic fertilizers caused distinct categories of shared potential hosts for TRGs. RDA analysis further indicated that the abundances of the shared potential hosts were affected by soil nutrients. These results suggested that the development of TRGs in soil following fertilization depended on the shared potential hosts with similar ecological niches between soil and fertilizers.