Epithelial tricellular junctions act as interphase cell shape sensors to orient mitosis.
Epithelial tricellular junctions act as interphase cell shape sensors to orient mitosis.
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DOI：
10.1038/nature16970
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发表时间：
2016-02-25
期刊：
影响因子：
64.8
通讯作者：
Bellaïche Y
中科院分区：
文献类型：
--
作者：
Bosveld F;Markova O;Guirao B;Martin C;Wang Z;Pierre A;Balakireva M;Gaugue I;Ainslie A;Christophorou N;Lubensky DK;Minc N;Bellaïche Y
细胞分裂方向沿着间期细胞长轴，这是世纪的古老Hertwig法则，对组织的增殖、形态发生、结构和力学有着深远的影响。在上皮组织中，间期细胞的形状受到细胞粘附、机械应力、相邻拓扑结构和平面极性途径的影响。在有丝分裂时，上皮细胞通常会变圆以确保染色体的可靠分离并促进形态发生。因此，组织中间期细胞形状感知的机制尚不清楚。我们发现，在果蝇上皮细胞中，三细胞连接（TCJ）定位微管力发生器，通过动力蛋白相关蛋白Mud独立于经典的Pins/Gαi途径定向细胞分裂。此外，当细胞在有丝分裂期间变圆时，TCJ充当空间地标，编码有关间期细胞形状各向异性的信息，以定向圆形有丝分裂细胞的分裂。最后，实验和模拟数据表明，形状和机械应变感测TCJ出现在上皮组织中的TCJ分布的一般几何属性。因此，除了它们作为上皮屏障结构的功能之外，TCJ还充当极性线索，促进上皮组织中的几何形状和机械感测。
The orientation of cell division along the interphase cell long-axis, the century old Hertwig’s rule, has profound roles in tissue proliferation, morphogenesis, architecture and mechanics. In epithelial tissues, the shape of the interphase cell is influenced by cell adhesion, mechanical stress, neighbour topology, and planar polarity pathways. At mitosis, epithelial cells usually round up to ensure faithful chromosome segregation and to promote morphogenesis. The mechanisms underlying interphase cell shape sensing in tissues are therefore unknown. We found that in Drosophila epithelia, tricellular junctions (TCJ) localize microtubule force generators, orienting cell division via the Dynein associated protein Mud independently of the classical Pins/Gαi pathway. Moreover, as cells round up during mitosis, TCJs serve as spatial landmarks, encoding information about interphase cell shape anisotropy to orient division in the rounded mitotic cell. Finally, experimental and simulation data show that shape and mechanical strain sensing by the TCJ emerge from a general geometric property of TCJ distributions in epithelial tissues. Thus, in addition to their function as epithelial barrier structures, TCJs serve as polarity cues promoting geometry and mechanical sensing in epithelial tissues.