Diversity, abundance and distribution of amoA-encoding archaea in deep-sea methane seep sediments of the Okhotsk Sea
Diversity, abundance and distribution of amoA-encoding archaea in deep-sea methane seep sediments of the Okhotsk Sea
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DOI：
10.1111/j.1574-6941.2010.00870.x
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发表时间：
2010-06-01
影响因子：
4.2
通讯作者：
Klotz, Martin G.
中科院分区：
文献类型：
--
作者：
Dang, Hongyue;Luan, Xi-Wu;Klotz, Martin G.
深海沉积物中编码amoa的古细菌（AEA）的生态学特征在很大程度上尚未解决。本文旨在研究鄂霍次克海（世界上最大的甲烷水合物储层之一）冷渗表层沉积物中古细菌群落的多样性、结构、分布和丰度，特别是古细菌组分。发现了多种古细菌16S rRNA基因序列，其中大多数与其他冷渗和富甲烷沉积物环境的序列有关。然而，amoA基因分析显示，AEA的多样性和丰度都很低。相关性分析表明，古菌amoA基因丰度与沉积物有机质含量相关。因此，携带amoa的古生菌可能利用有机物为生。某些古细菌amoA序列与原产于深海热液喷口环境的GenBank序列的关联表明，相关AEA在鄂霍次克海现代深海冷沉积中具有广泛的温度适应或嗜热进化史。氨氧化菌对AEA的优势可能表明细菌在鄂霍次克海冷渗沉积物的硝化作用中起重要作用。
The ecological characteristics of amoA-encoding archaea (AEA) in deep-sea sediments are largely unsolved. This paper aimed to study the diversity, structure, distribution and abundance of the archaeal community and especially its AEA components in the cold seep surface sediments of the Okhotsk Sea, a marginal sea harboring one of the largest methane hydrate reservoirs in the world. Diverse archaeal 16S rRNA gene sequences were identified, with the majority being related to sequences from other cold seep and methane-rich sediment environments. However, the AEA diversity and abundance were quite low as revealed by amoA gene analyses. Correlation analysis indicates that the abundance of the archaeal amoA genes was correlated with the sediment organic matter content. Thus, it is possible that the amoA-carrying archaea here might utilize organic matter for a living. The affiliation of certain archaeal amoA sequences to the GenBank sequences originally obtained from deep-sea hydrothermal vent environments indicated that the related AEA either have a wide range of temperature adaptation or they have a thermophilic evolutionary history in the modern cold deep-sea sediments of the Okhotsk Sea. The dominance of ammonia-oxidizing bacteria over AEA may indicate that bacteria play a significant role in nitrification in the Okhotsk Sea cold seep sediments.