Genetic characterization of HIV-1 BC recombinants and evolutionary history of the CRF31_BC in Southern Brazil
Genetic characterization of HIV-1 BC recombinants and evolutionary history of the CRF31_BC in Southern Brazil
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DOI：
10.1016/j.meegid.2009.01.008
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发表时间：
2009-07-01
影响因子：
3.2
通讯作者：
Guimares, Monick Lindenmeyer
中科院分区：
文献类型：
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作者：
Bittencourt Passaes, Caroline Pereira;Bello, Gonzalo;Guimares, Monick Lindenmeyer
为了评价巴西南部HIV-1 BC重组体的重组特征和进化史，对1998年至2006年在波尔图阿莱格雷市（南里奥格兰德州）采集的81株先前亚型为C（env-gp 120/C2 V3）的分离株进行了蛋白酶-逆转录酶（pr/rt）、整合酶和gp 41基因组区域的筛选。进行详细的系统发育、bootscan和信息位点分析以追踪亚型分类。巴西CRF31_BC流行病的进化速率和发散时间估计使用贝叶斯马尔可夫链蒙特卡罗框架。对四个靶区域的分析确定：43个分离株为“纯”C亚型，23个为CRF31_BC，15个为独特的BC重组形式（URFs_BC）。纯化断裂点主要位于rt基因，仅分析该区域即可检测到100%的重组样品。大多数URFs_BC（86.7%）含有小的B亚型片段（
To evaluate the recombination profiles and evolutionary history of HIV-1 BC recombinants in Southern Brazil, 81 isolates collected in the city of Porto Alegre (Rio Grande do Sul State) from 1998 to 2006 previously subtyped as C (env-gp120/C2V3) were screened in the protease-reverse transcriptase (pr/rt), integrase and gp41 genomic regions. Detailed phylogenetic, bootscan and informative site analyses were performed to trace the subtype classification. The evolutionary rate and divergence time of the Brazilian CRF31_BC epidemic were estimated using a Bayesian Markov Chain Monte Carlo framework. Analysis of the four target regions identified: 43 isolates as "pure" subtype C, 23 as CRF31_BC, and 15 as unique BC recombinant forms (URFs_BC). Recombination breakpoints were mainly localized in the rt gene and 100% of the recombinant samples could be detected analyzing only this region. Most URFs_BC (86.7%) contained small subtype B fragments (