DIFFERENCES IN GLYCOGEN-METABOLISM IN ASTROGLIA-RICH PRIMARY CULTURES AND SORBITOL-SELECTED ASTROGLIAL CULTURES DERIVED FROM MOUSE-BRAIN
DIFFERENCES IN GLYCOGEN-METABOLISM IN ASTROGLIA-RICH PRIMARY CULTURES AND SORBITOL-SELECTED ASTROGLIAL CULTURES DERIVED FROM MOUSE-BRAIN
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DOI：
10.1002/glia.440080302
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发表时间：
1993-07-01
期刊：
影响因子：
6.2
通讯作者：
HAMPRECHT, B
中科院分区：
文献类型：
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作者：
DRINGEN, R;HAMPRECHT, B
最近，通过化学选择，用山梨糖醇代替葡萄糖作为培养基，从鼠脑富含星形胶质细胞的原代培养物中获得纯星形胶质细胞培养物成为可能。脑内糖原降解酶糖原磷酸化酶定位于星形胶质细胞和室管膜细胞。鉴于这一事实，似乎有必要研究葡萄糖和其他己糖对这些缺乏其他细胞类型影响的培养物中糖原代谢的影响，与含有几种类型细胞的富含星形胶质细胞的原代培养物相比。山梨醇喂养的选定培养物和缺乏葡萄糖的富含星形胶质细胞的原代培养物所含糖原少于葡萄糖喂养原代培养物所含糖原的10%。在与葡萄糖孵育期间，所选培养物和无葡萄糖原代培养物的糖原含量增加了一个数量级以上。然而，并不是所有的细胞都有糖原积累。两种类型的培养中糖原补充的时间过程相似，尽管在选定的培养中达到的最高水平是富含星形胶质细胞的原代培养的3倍。这种差异可能是由于选择培养的糖原合成酶和糖原磷酸化酶的最大活性之比是未选择培养的两倍。在葡萄糖剥夺过程中，两种培养体系中的糖原含量都有所降低，在15分钟（原代培养）和45分钟（选定培养）时达到最大含量的一半。这两种类型的培养也可以利用甘露糖合成糖原和生产乳酸。相反，果糖或半乳糖是生成糖原的较差底物，或者根本没有底物。综上所述，与富含星形胶质细胞的原代培养相比，无非星形胶质细胞和葡萄糖喂养后糖原含量高，使得纯星形胶质细胞培养成为研究星形胶质细胞糖原调节和功能的更好系统。
Recently it has become possible by chemical selection using sorbitol instead of glucose in the culture medium to produce pure astroglial cultures from astroglia-rich primary cultures from mouse brain. The glycogen-degrading enzyme glycogen phosphorylase in brain is localized in astrocytes and ependymal cells. In view of this fact it appeared necessary to study the influence of glucose and other hexoses on the glycogen metabolism in these cultures lacking the influence of other cell types in comparison to the astroglia-rich primary cultures containing several types of cells. The sorbitol-fed selected cultures and the glucose-deprived astroglia-rich primary cultures contain less than 10% of the glycogen encountered in glucose-fed primary cultures. During incubation with glucose the glycogen content of the selected cultures and the glucose-deprived primary cultures increases by more than one order of magnitude. Nevertheless, not all cells are found to have accumulated glycogen. The time course of the replenishment of glycogen is similar in both types of culture, although maximal levels reached in the selected cultures are 3 times those in the astroglia-rich primary cultures. This difference might be explained by the fact that the ratio of the maximal activities of glycogen synthase and glycogen phosphorylase in selected cultures was found to be twice that in the unselected cultures. During glucose deprivation the glycogen content is reduced in both culture systems with half-maximal contents being reached at 15 min (primary culture) and 45 min (selected culture). Both types of culture can also utilize mannose for the synthesis of glycogen and the production of lactate. In contrast, fructose or galactose are poor substrates, or none at all, for the generation of glycogen. In conclusion, the absence of non-astroglial cells and the high glycogen content after glucose feeding makes pure astroglial cultures an even better system for investigating the regulation and the function of astrocytic glycogen than astroglia-rich primary cultures.