ACCESSORY CHOLERA ENTEROTOXIN (ACE), THE 3RD TOXIN OF A VIBRIO-CHOLERAE VIRULENCE CASSETTE
ACCESSORY CHOLERA ENTEROTOXIN (ACE), THE 3RD TOXIN OF A VIBRIO-CHOLERAE VIRULENCE CASSETTE
复制标题
DOI：
10.1073/pnas.90.11.5267
复制
发表时间：
1993-06-01
影响因子：
11.1
通讯作者：
KAPER, JB
中科院分区：
文献类型：
--
作者：
TRUCKSIS, M;GALEN, JE;KAPER, JB
霍乱弧菌通过肠道分泌源霍乱毒素的阐述而导致潜在的致命性疾病霍乱。霍乱弧菌的第二种毒素Zot通过改变细胞间的紧密连接来降低肠道组织的抵抗力。本文报道了霍乱弧菌的第三种毒素--Ace(副霍乱肠毒素)。血管紧张素转换酶可增加Ussing腔的短路电流，并导致结扎的兔回肠的液体分泌。预测的Ace蛋白序列与真核细胞离子转运ATPase有惊人的相似性，包括囊性纤维化基因的产物。编码Ace的基因位于Zot和霍乱毒素编码基因的上游。Ctx、zot和ace基因位于染色体的一个动态区段，由霍乱弧菌“毒力盒”组成。
Vibrio cholerae causes the potentially lethal disease cholera through the elaboration of the intestinal secretogen cholera toxin. A second toxin of V. cholerae, Zot, decreases intestinal tissue resistance by modifying intercellular tight junctions. In this report, a third toxin of V. cholerae, Ace (accessory cholera enterotoxin), is described. Ace increases short-circuit current in Ussing chambers and causes fluid secretion in ligated rabbit ileal loops. The predicted protein sequence of Ace shows striking similarity to eukaryotic ion-transporting ATPases, including the product of the cystic fibrosis gene. The gene encoding Ace is located immediately upstream of the genes encoding Zot and cholera toxin. The ctx, zot, and ace genes, which are located on a dynamic sector of the chromosome, comprise a V. cholerae ''virulence cassette.''