Identification of Factors Complicating Bioluminescence Imaging
Identification of Factors Complicating Bioluminescence Imaging
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DOI：
10.1021/acs.biochem.8b01303
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发表时间：
2019-03-26
期刊：
影响因子：
2.9
通讯作者：
Ai, Hui-wang
中科院分区：
文献类型：
--
作者：
Yeh, Hsien-Wei;Wu, Tianchen;Ai, Hui-wang
体内生物发光成像（BLI）已成为一种标准的，非侵入性的成像模式，用于以下基因表达或蛋白质和细胞在活体动物的命运。目前，在实验室中使用的生物发光报告基因主要是两个主要的荧光素酶家族的衍生物：ATP依赖性昆虫荧光素酶和ATP非依赖性海洋荧光素酶。使用不同的生物发光报告基因，如Akaluc和Antareas2的实验结果不一致，已被报道。在此，我们重新检查了几个实验设置中的不一致性，并确定了导致观察到的差异的因素，如ATP依赖性、血清中的稳定性和酶的分子大小。我们希望这项研究将使研究界意识到这些因素，并通过考虑每个生物发光记者的性质，促进更准确地解释BLI数据。
In vivo bioluminescence imaging (BLI) has become a standard, non-invasive imaging modality for following gene expression or the fate of proteins and cells in living animals. Currently, bioluminescent reporters used in laboratories are mostly derivatives of two major luciferase families: ATP-dependent insect luciferases and ATP-independent marine luciferases. Inconsistent results of experiments using different bioluminescent reporters, such as Akaluc and Antareas2, have been reported. Herein, we re-examined the inconsistency in several experimental settings and identified the factors, such as ATP dependency, stability in serum, and molecular sizes of luciferases, that contributed to the observed differences. We expect this study will make the research community aware of these factors and facilitate more accurate interpretation of BLI data by considering the nature of each bioluminescent reporter.