Reconstruction of the primate mandible with a combination graft of recombinant human bone morphogenetic protein-2 and bone marrow
Reconstruction of the primate mandible with a combination graft of recombinant human bone morphogenetic protein-2 and bone marrow
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DOI：
10.1053/joms.2001.19286
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发表时间：
2001-01-01
影响因子：
1.9
通讯作者：
Enomoto, S
中科院分区：
文献类型：
--
作者：
Seto, I;Asahina, I;Enomoto, S
目的：本研究评估重组人骨形态发生蛋白-2（rhRMP-2）是否可用于再生的切除部分的下颌骨在灵长类model.Materials和方法：节段性骨缺损手术创建在日本猴的下颌骨。将rhBMP-2悬浮于聚乙醇酸-共-乳酸（PGLA）溶液中并冻干以制备BMP/PGLA复合物。将rhBMP-2/PGLA复合物和自体骨髓以3：0或2：1（vol：vol）的比例分别植入3只猴的骨缺损中。2.5.0.5将骨髓或单独的PGLA各自植入1只猴作为对照。术后16周处死动物，进行放射学和组织学评价。结果：单纯骨髓移植成功重建下颌骨，而rhBMP-2/PGLA复合物移植仅显示少量骨形成。结论：rhBMP-2/PGLA与骨髓联合移植修复下颌骨节段性缺损，所需骨髓量少，是一种可靠的修复方法。(C)2001年美国口腔颌面外科医师协会。
Purpose: This study evaluated whether recombinant human bone morphogenetic protein-2 (rhRMP-2) can be used to regenerate a resected part of the mandible in a primate model.Materials and Methods: Segmental bone defects were created surgically in the mandible of Japanese monkeys. rhBMP-2 was suspended in a solution of polyglycolic co-lactic acid (PGLA) and lyophilized to make a BMP/PGLA complex. The rhBMP-2/PGLA complex and autogenous bone marrow in ratios of 3:0, 2.5.0.5, or 2:1 (vol:vol) were each implanted into the bone defects in 3 monkeys. Bone marrow or PGLA alone were each implanted in I monkey as a control. The animals were killed 16 weeks after surgery, followed by radiologic and histologic evaluation.Results: The implantation of bone marrow alone succeeded in reconstruction of the mandible, but the implantation of the rhBMP-2/PGLA complex showed only a small amount of bone formation. The combination graft of rhBMP-2/PGLA and bone marrow resulted in a greater degree of bone formation; especially the 2:1 combination showed the same result as only bone marrow implantation.Conclusion: The combination graft of rhBMP-2 and bone marrow, which requires only a small amount of bone marrow, was a reliable method for reconstruction of mandibular segmental defects in this animal model. (C) 2001 American Association of Oral and Maxillofacial Surgeons.