Effects of fluid flow on intracellular calcium in bovine articular chondrocytes.
Effects of fluid flow on intracellular calcium in bovine articular chondrocytes.
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流体流动对牛关节软骨细胞内钙的影响。
DOI：
10.1152/ajpcell.1997.273.1.c30
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Donahue,HJ
中科院分区：
文献类型：
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作者：
Yellowley,CE;Jacobs,CR;Li,Z;Zhou,Z;Donahue,HJ
流体诱导的剪应力导致培养的牛关节软骨细胞(BAC)发生各种形态和代谢变化。然而，流动信号被转化为生物反应的机制尚不清楚。因此，我们研究了液体流动对BAC细胞内钙离子浓度([Ca~(2+)]i)的影响。负载Fura-2的细胞暴露在平行板流室中，以9、18和34ml/min的速度稳定和脉动(0.5赫兹)流动。在对流量的反应中，表现为[Ca~(2+)]i升高的细胞百分比显著增加且呈流量依赖性，但对[Ca~(2+)]_i的反应幅度没有影响。[Ca~(2+)]_i对稳态流和脉动流的反应无显著差异。细胞内钙平均反应值为26.2+/-1.6(9ml/min)至38.0+/-6.8 nM(34ml/min)，高于基础[Ca~(2+)]i(81.3/-24.1 nM，n=90)。细胞外钙离子的去除或Gd3+的加入显著降低了细胞的反应百分率，提示钙离子的内流可能通过机械敏感通道导致细胞内钙离子的升高。我们的数据表明，流体诱导的细胞内钙离子的动员可能参与了软骨细胞传递机械负荷的机制。
Fluid flow-induced shear stress results in a variety of morphological and metabolic changes in cultured bovine articular chondrocytes (BAC). However, the mechanism by which the flow signal is transduced into a biological response is unknown. Therefore, we investigated the effects of fluid flow on intracellular Ca2+ concentration ([Ca2+]i) in BAC. Cells loaded with fura 2 were exposed to steady and pulsatile (0.5 Hz) flow at 9, 18, and 34 ml/min in a parallel-plate flow chamber. In response to flow, there was a significant and flow rate-dependent increase in the percentage of cells showing a rise in [Ca2+]i, but no effect on the [Ca2+]i response amplitude. There was no significant difference between the [Ca2+]i responses to steady and pulsatile flow. Mean intracellular Ca2+ response values ranged between 26.2 +/- 1.6 (9 ml/min) and 38.0 +/- 6.8 nM (34 ml/min) above basal [Ca2+]i (81.3 +/- 24.1 nM, n = 90). Removal of extracellular Ca2+ or addition of Gd3+ significantly reduced the percentage of cells responding, suggesting that influx of Ca2+, possibly through mechanosensitive channels, contributes to the rise in intracellular Ca2+. Our data suggest fluid flow-induced mobilization of intracellular Ca2+ may contribute to the mechanism by which mechanical loads are transduced by chondrocytes.