The different replication between nonenveloped and quasi-enveloped hepatitis E virus
The different replication between nonenveloped and quasi-enveloped hepatitis E virus
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DOI：
10.1002/jmv.27121
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发表时间：
2021-06-10
影响因子：
12.7
通讯作者：
Huang, Fen
中科院分区：
文献类型：
--
作者：
Ji, Hanbin;Chen, Shuangfeng;Huang, Fen
戊型肝炎病毒（HEV）是病毒性肝炎的主要病原体。然而，对 HEV 生命周期的了解是有限的。在本研究中，细胞分别用无包膜HEV（源自粪便或胆汁）或准包膜HEV（源自连续传代后的细胞培养物，eHEV）感染，并通过共聚焦荧光显微镜观察以研究HEV的生命周期。 HEV 在接种后 6 小时 (hpi) 完成结合并进入宿主细胞。接种 eHEV 的细胞比接种无包膜 HEV 的细胞表现出较低的感染性。新合成的子代病毒粒子从 48 hpi 开始释放到细胞培养物的上清液中。实时定量聚合酶链反应（qRT-PCR）和蛋白质印迹分析结果表明，上清液的子代病毒即使在连续传代五次后仍具有感染性。这些结果显示了无包膜 HEV 和 eHEV 之间的显着差异，这将为 HEV 复制周期提供新的见解。 HEV的高效细胞培养将促进抗HEV药物和疫苗的开发。
Hepatitis E virus (HEV) is the major pathogen of viral hepatitis. However, the understanding of the HEV life cycle is limited. In the present study, cells were separately infected with nonenveloped HEV (derived from feces or bile) or quasi-enveloped HEV (derived from the cell culture after serial passages, eHEV) and observed by confocal fluorescence microscopy to investigate the life cycle of HEV. HEV finished its binding and entry into host cells at first 6 h postinoculation (hpi). Cells inoculated with eHEV showed less infectivity than cells inoculated with nonenveloped HEV. Newly synthesized progeny virions were released into the supernatant of cell cultures from 48 hpi. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis results showed that the supernatant's progeny viruses were infectious even after five serial passages. These results show the significant difference between nonenveloped HEV and eHEV, which will provide novel insights into the HEV replication cycle. The efficient cell culture of HEV will promote the development of anti-HEV drugs and vaccines.