Transporter-Mediated Nuclear Entry of Jasmonoyl-Isoleucine Is Essential for Jasmonate Signaling
Transporter-Mediated Nuclear Entry of Jasmonoyl-Isoleucine Is Essential for Jasmonate Signaling
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DOI：
10.1016/j.molp.2017.01.010
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发表时间：
2017-05-01
期刊：
影响因子：
27.5
通讯作者：
Liu, Pei
中科院分区：
文献类型：
--
作者：
Li, Qingqing;Zheng, Jian;Liu, Pei
为了通过直接响应激素浓度来控制基因表达，动物和植物细胞都利用了类似的机制来感知细胞核中的小分子激素。这些激素进入核的过程是主动转运还是被动扩散（如传统假设）通过核孔复合体，仍然是个谜。在这里，我们鉴定并表征了拟南芥中的茉莉酸转运蛋白 AtJAT1/AtABCG16，它在核膜和质膜上表现出意想不到的双重定位。我们发现 AtJAT1/AtABCG16 通过介导茉莉酸 (JA) 的细胞流出和茉莉酰异亮氨酸 (JA-Ile) 的核流入来控制茉莉酸植物激素的细胞质和细胞核分配，并且对于维持关键的核 JA-Ile 浓度以激活 JA 信号传导至关重要。这些结果表明，转运蛋白介导的小激素分子进入核是调节核激素信号传导的新机制。我们的研究结果为开发针对小分子进入核的药剂提供了途径。
To control gene expression by directly responding to hormone concentrations, both animal and plant cells have exploited comparable mechanisms to sense small-molecule hormones in nucleus. Whether nuclear entry of these hormones is actively transported or passively diffused, as conventionally postulated, through the nuclear pore complex, remains enigmatic. Here, we identified and characterized a jasmonate transporter in Arabidopsis thaliana, AtJAT1/AtABCG16, which exhibits an unexpected dual localization at the nuclear envelope and plasma membrane. We show that AtJAT1/AtABCG16 controls the cytoplasmic and nuclear partition of jasmonate phytohormones by mediating both cellular efflux of jasmonic acid (JA) and nuclear influx of jasmonoyl-isoleucine (JA-Ile), and is essential for maintaining a critical nuclear JA-Ile concentration to activate JA signaling. These results illustrate that transporter-mediated nuclear entry of small hormone molecules is a new mechanism to regulate nuclear hormone signaling. Our findings provide an avenue to develop pharmaceutical agents targeting the nuclear entry of small molecules.