Repair of rabbit radial bone defects using true bone ceramics combined with BMP-2-related peptide and type I collagen
Repair of rabbit radial bone defects using true bone ceramics combined with BMP-2-related peptide and type I collagen
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DOI：
10.1016/j.msec.2010.07.011
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发表时间：
2010-10-12
影响因子：
7.9
通讯作者：
Yang, Shuhua
中科院分区：
文献类型：
--
作者：
Li, Jingfeng;Lin, Zhenyu;Yang, Shuhua
理想的骨移植材料是具有良好的生物相容性、生物降解性、骨传导性和骨诱导性的材料。本研究利用模拟体液(SBF)将一种新型的人工合成的BMP-2相关多肽(命名为P24)引入到基于烧结牛骨或真骨陶瓷(TBC)和I型胶原(TBC/I型胶原)的仿生支架中。采用扫描电子显微镜、能谱仪和X射线衍射仪对P24/TBC/I型胶原复合材料表面钙磷比为1.63的羟基磷灰石晶体矿化进行了观察。体外细胞黏附率测定结果显示，P24/TBC/I型胶原复合体的贴壁率明显高于TBC/I型胶原复合体。新西兰大白兔造成单侧10 mm节段性骨缺损，随机植入3组生物材料(A组：P24/TBC/I型胶原复合体；B组：TBC/I型胶原复合体；C组：单纯TBC)。根据放射学评价和组织学检查，P24/TBC/I型胶原复合材料种植体明显促进骨生长，从而证实了与TBC/I型胶原复合材料和TBC单独植入相比，P24/TBC/I型胶原复合材料具有更高的骨愈合速度。结论：BMP-2相关肽P24可诱导TBC/I型胶原复合材料表面磷酸钙晶体成核。人工合成的BMP-2相关多肽负载的TBC/I型胶原复合材料是一种很有前途的骨组织工程支架材料。(C)2010爱思唯尔B.V.保留所有权利。
An ideal bone graft material is the one characterized with good biocompatibility, biodegradation, osteoconductivity and osteoinductivity. In this study, a novel synthetic BMP-2-related peptide (designated P24) corresponding to residues of the knuckle epitope of BMP-2 was introduced into a biomimetic scaffold based on sintered bovine bone or true bone ceramics (TBC) and type I collagen (TBC/collagen I) using a simulated body fluid (SBF). Hydroxylapatite crystal mineralization with a Ca/P molar ratio of 1.63 was observed on the surface of P24/TBC/collagen I composite by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD) techniques. Cell adhesion rate evaluation of bone marrow stromal cells (BMSCs) seeded on materials in vitro showed that the percentage of cells attached to P24/TBC/collagen I composite was significantly higher than that of the TBC/collagen I composite. A 10 mm unilateral segmental bone defect was created in the radius of New Zealand white rabbits and randomly implanted with three groups of biomaterials (Group A: P24/TBC/collagen I composite; Group B: TBC/collagen I composite and Group C: TBC alone). Based on radiographic evaluation and histological examination, the implants of P24/TBC/collagen I composite significantly stimulated bone growth, thereby confirming the enhanced rate of bone healing compared with that of TBC/collagen I composite and TBC alone. It was concluded that BMP-2-related peptide P24 could induce nucleation of calcium phosphate crystals on the surface of TBC/collagen I composite. The TBC/collagen I composite loaded with the synthetic BMP-2-related peptide is a promising scaffold biomaterial for bone tissue engineering. (C) 2010 Elsevier B.V. All rights reserved.