Probiotic VSL#3-induced TGF-β ameliorates food allergy inflammation in a mouse model of peanut sensitization through the induction of regulatory T cells in the gut mucosa
Probiotic VSL#3-induced TGF-β ameliorates food allergy inflammation in a mouse model of peanut sensitization through the induction of regulatory T cells in the gut mucosa
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DOI：
10.1002/mnfr.201300028
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发表时间：
2013-12-01
影响因子：
5.2
通讯作者：
Di Felice, Gabriella
中科院分区：
文献类型：
--
作者：
Barletta, Bianca;Rossi, Giacomo;Di Felice, Gabriella
范围在食物过敏中，花生过敏经常与严重的过敏反应有关。在需要安全和有效的治疗策略，益生菌可能会考虑其免疫调节特性的基础上。本研究的目的是研究益生菌VSL#3口服补充对花生过敏小鼠模型中Th 2炎症和过敏反应的影响中涉及的免疫介质。方法和结果通过促进空肠粘膜和肠系膜淋巴结中的调节反应，通过ELISA、实时PCR、组织学和免疫组织化学分析进行评价。益生菌诱导的TGF-β通过诱导表达FOXP 3和/或潜伏相关肽的调节性T细胞介导其保护作用，如通过在攻击前一天用中和单克隆抗体在VSLI #3处理的小鼠中体内阻断TGF-β所证明的。能够减少花生致敏小鼠模型中与食物过敏反应相关的Th 2炎症。TGF-β通过诱导/维持表达FOXP 3和/或潜伏相关肽的调节性T细胞起作用。益生菌补充剂可能是治疗成人食物过敏的有效和安全的策略。
ScopeAmong food allergies, peanut allergy is frequently associated with severe anaphylactic reactions. In the need for safe and effective therapeutic strategies, probiotics may be considered on the basis of their immunomodulatory properties. The aim of the present study was to investigate the immunological mediators involved in the effects of probiotic VSL#3 oral supplementation on Th2 inflammation and anaphylaxis in a mouse model of peanut allergy.Methods and resultsVSL#3 supplementation to peanut-sensitized mice was effective in ameliorating anaphylaxis and Th2-mediated inflammation, by promoting regulatory responses in the jejunum mucosa and in the mesenteric lymph node, as evaluated by ELISA, real-time PCR, histologic, and immunohistochemical analysis. Probiotic-induced TGF- mediates its protective effects through the induction of regulatory T cells expressing FOXP3 and/or latency-associated peptide, as proven by in vivo blockade of TGF- in VSL#3-treated mice with a neutralizing monoclonal antibody one day before challenge.ConclusionTGF-, induced in the gut by VSL#3 supplementation, is capable of reducing the Th2 inflammation associated with food anaphylaxis in a mouse model of peanut sensitization. TGF- acts through the induction/maintenance of regulatory T cells expressing FOXP3 and/or latency-associated peptide. Probiotics supplementation may represent an effective and safe strategy for treating food allergies in adult population.