MUCOSUBSTANCES OF THE RAT GASTRIC MUCOSA STUDIED BY MEANS OF SEVERAL HISTOCHEMICAL METHODS
MUCOSUBSTANCES OF THE RAT GASTRIC MUCOSA STUDIED BY MEANS OF SEVERAL HISTOCHEMICAL METHODS
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多种组织化学方法研究大鼠胃粘膜粘膜物质
DOI：
10.1267/ahc.14.534
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发表时间：
1981
影响因子：
2.4
通讯作者：
F. Murata
中科院分区：
文献类型：
--
作者：
T. Suganuma;Shintaro T. Suzuki;S. Tsuyama;F. Murata
本文用~（35）S-硫酸钠放射自显影、光镜组织化学、过碘酸硫代碳酰肼银蛋白（PA-TCH-SP）法和凝集素（麦胚凝集素）铁蛋白结合物染色法研究了大鼠胃粘膜内粘液物质的分布。组织化学染色显示，浅表覆盖上皮含有大量的中性粘液物质，而小窝和峡部粘液细胞产生唾液酸粘蛋白和硫酸粘蛋白。在胃底凹和峡部以及幽门腺中观察到35 S的主动掺入。颈粘液细胞含有唾液粘蛋白。PA-TCH-SP法染色覆盖上皮、小凹和峡部粘液细胞上皮及颈粘液细胞。所有参与胃粘膜颗粒物质形成的细胞器均用此法染色。颈粘液细胞胞质内广泛分布圆形PA-TCH-SP阳性颗粒。随着小凹上皮的成熟，颗粒的大小增加。覆盖上皮的顶端部分仅被大的圆形至椭圆形颗粒占据。PA-TCH-SP染色后，许多颗粒呈二分结构，仅在颗粒的周边区域观察到反应产物。相反，随着这些上皮细胞的成熟，其他细胞器官的发育变得不那么突出。用凝集素（麦胚凝集素）-铁蛋白结合物染色后，这些粘液细胞的分泌颗粒具有不同的染色模式。在覆盖上皮中，缀合物仅染色颗粒的周边区域。
Distribution of mucosubstances in the rat gastric mucosa was studied by radioautography using 35S-sodium sulfate, several light microscopic histochemical procedures, the periodic acid thiocarbohydrazide silver proteinate (PA-TCH-SP) method and postembedding staining with Iectin (wheat germ agglutinin) ferritin conjugates. Histochemical staining revealed that the superficial covering epithelium contained a large amount of neutral mucosubstances, whereas the foveolar and isthmus mucous cells produced both sialomucins and sulfomucins. Active incorporation of 35S was observed in the fundic foveolus and isthmus as well as the pyloric gland. The mucous neck cells contained sialomucin. The PA-TCH-SP method stained the covering epithelium, the epithelium of the foveolar and isthmus mucous cells and the mucous neck cells. All cell organellae which took part in the formation of granule substances in the gastric mucosa were stained by this method. The cytoplasm of the mucous neck cells was extensively occupied with round PA-TCH-SP positive granules. As the epithelium of the pits matured, the granules increased in size. The apical portion of the covering epithelium was exclusively occupied with large round to oval granules. Many of them showed a bipartite structure after PA-TCH-SP staining, reaction products were observed only in the peripheral region of these granules. Conversely, the development of other cell organellae became less prominent as these epithelia matured. Postembedding staining with lectin (wheat germ agglutinin)-ferritin conjugates stained secretory granules of all these mucous cells with different staining patterns. In the covering epithelium, the conjugate stained only the peripheral region of the granules.