Symptom pathogenesis during acute influenza: Interleukin-6 and other cytokine responses
Symptom pathogenesis during acute influenza: Interleukin-6 and other cytokine responses
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DOI：
10.1002/jmv.1045
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发表时间：
2001-07-01
影响因子：
12.7
通讯作者：
Hayden, FG
中科院分区：
文献类型：
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作者：
Kaiser, L;Fritz, RS;Hayden, FG
在鼻接种引发的实验性人流感感染中，病毒复制、发热和症状的程度与鼻咽灌洗液中各种细胞因子的水平相关。我们的目的是评估自然发生的急性流感患者的这些关系。研究了持续时间少于36小时的流感病毒培养阳性患者。在入组时和第2、4、6和8天收集鼻咽冲洗液，用于定量病毒分离和IL-6、TNF-α、INF-α、INF-γ和IL-10测定。处理入组时以及第2天和第6天采集的血样，以评估血浆细胞因子和循环流感RNA。患者接受奥司他韦或安慰剂治疗5天。我们评估了治疗前鼻咽灌洗液或血液中细胞因子水平与病毒滴度、症状严重程度和发热之间的相关性。研究了16名成年受试者（中位年龄为22岁），在这一小组患者中，安慰剂组和奥司他韦组患者在病毒复制或细胞因子测定方面没有发现显著差异。因此，汇总了所有16例受试者的数据进行分析。入组时，从鼻咽洗液中培养甲型流感病毒，中位滴度为4.8 log（10）TCID（50）/ml洗液。病毒滴度与症状评分（P=0.006）和体温值（P
In experimental human influenza infection initiated by nasal inoculation, the magnitude of viral replication, fever, and symptoms correlate with nasopharyngeal lavage fluid levels of various cytokines. Our aim was to assess these relationships in patients with naturally occurring acute influenza. Patients with culture-positive influenza illness of less than 36 hr of duration were studied. Nasopharyngeal washing were collected at enrollment and on Day 2, 4, 6 and 8 for quantitative virus isolation and IL-6, TNF-alpha, INF-alpha, INF-gamma and IL-10 determinations. Blood samples collected at entry and on Day 2 and 6 were processed to assess plasma cytokines and circulating influenza RNA. Patients received either oseltamivir or placebo for 5 days. We assessed the correlation between nasopharyngeal lavage fluid or blood levels of cytokines before treatment and viral titers, symptom severity and fever. Sixteen adult subjects (median age of 22 years) were studied, in this small group of patients no significant differences between placebo and oseltamivir patients were found in viral replication or measures of cytokines. Thus the data for all 16 subjects were pooled for analysis. At entry, influenza A viruses were cultured from nasopharyngeal washes at a median titer of 4.8 log(10)TCID(50)/ml of wash. Viral titers correlated positively with symptom score (P=0.006) and temperature values (P