Endosialin is expressed on stromal fibroblasts and CNS pericytes in mouse embryos and is downregulated during development
Endosialin is expressed on stromal fibroblasts and CNS pericytes in mouse embryos and is downregulated during development
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DOI：
10.1016/j.modgep.2006.07.006
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发表时间：
2007-01-01
影响因子：
1.2
通讯作者：
Isacke, Clare M.
中科院分区：
文献类型：
--
作者：
MacFadyen, John;Savage, Kay;Isacke, Clare M.
内皮唾液酸被指定为肿瘤内皮标志物I（TEM 1）的别名，因为与正常内皮相比，它被鉴定为肿瘤内皮中高度上调的基因转录物。因此，人们对内唾液酸蛋白作为癌症治疗中的潜在治疗靶标感兴趣。然而，关于肿瘤血管表达的性质存在相互矛盾的报道，声音证据表明内皮唾液酸蛋白表达于血管周细胞而不是内皮细胞本身。为了解决这个问题，我们分析了内皮唾液酸蛋白在小鼠胚胎，新生幼崽和成年小鼠中的表达。在胚胎中，内皮唾液素主要在整个间充质的基质成纤维细胞上表达，但也在发育中的脉管系统上观察到表达。当通过共聚焦显微镜分析时，血管上的内唾液酸蛋白不与表达CD 31的内皮细胞共定位。相反，内皮唾液酸蛋白仅限于密切相关的血管周细胞，也表达周细胞标记物NG2。最后，成纤维细胞和周细胞表达的内皮唾液酸动态变化过程中的发展，并成为高度限制在成年小鼠组织。在活跃的组织重塑和新血管形成的位点中，这种内唾液酸蛋白表达的演变图像在肿瘤生长、血管生成和转移中具有意义。(c)2006 Elsevier B.V.保留所有权利。
Endosialin has been assigned the alternate name of tumour endothelial marker I (TEM1) due to its identification as a highly upregulated gene transcript in tumour endothelium compared to normal endothelium. As a consequence there is interest in endosialin as a potential therapeutic target in cancer treatment. However, there are conflicting reports over the nature of vascular expression in tumours with sonic evidence that endosialin is expressed on perivascular pericytes rather than the endothelial cells themselves. To address this, we have analysed the expression of endosialin in mouse embryos, newborn pups and adults. In the embryo endosialin is predominantly expressed on stromal fibroblasts throughout the mesenchyme but expression is also observed on the developing vasculature. When analysed by confocal microscopy endosialin on vessels does not colocalise with endothelial cells expressing CD31. Rather, endosialin is restricted to closely associated perivasular cells that also express the pericyte marker NG2. Finally, the fibroblast and pericyte expression of endosialin changes dynamically during development and becomes highly restricted in adult mouse tissues. This evolving picture of endosialin expression in sites of active tissue remodelling and neovascularisation has implications in tumour growth, angiogenesis and inetastasis. (c) 2006 Elsevier B.V. All rights reserved.