Acanthamoeba discriminates internally between digestible and indigestible particles.
Acanthamoeba discriminates internally between digestible and indigestible particles.
复制标题
DOI：
10.1083/jcb.97.2.317
复制
发表时间：
1983-08
影响因子：
7.8
通讯作者：
Olszewski, T E
中科院分区：
文献类型：
--
作者：
Bowers, B;Olszewski, T E
研究了棘阿米巴在吞噬过程中区分营养性酵母颗粒和非营养性塑料珠的能力。当细胞被允许吞噬酵母到容量时，内吞作用停止，随后的颗粒（酵母或珠）的呈现不会导致进一步的摄取。相比之下，当细胞被允许吞噬塑料珠，并给予第二剂量的颗粒（酵母或珠）时，细胞排出内部颗粒，并吸收新的颗粒。通过广泛的化学交联使酵母变得难以消化，其吸收速度与未处理的酵母相似，但是，像珠子一样，当第二剂量的颗粒出现时，它们被排出体外。结果表明，在含有酵母的液泡和含有塑料珠的液泡之间存在内部区别，并且它们与液泡中能够被消化的物质的存在可以防止胞吐的假设是一致的。
The capacity of Acanthamoeba to distinguish nutritive yeast particles from non-nutritive plastic beads during phagocytosis was investigated. When cells were allowed to phagocytose yeast to capacity, endocytosis stopped and subsequent presentation of particles (either yeast or beads) did not result in further uptake. By contrast, when cells were allowed to phagocytose plastic beads to capacity and a second dose of particles was presented (either yeast or beads), the cells exocytosed the internal particles and took up new ones. Yeast rendered indigestible by extensive chemical cross-linking were taken up at rates similar to those of untreated yeast, but, like beads, they were exocytosed when a second dose of particles was presented. The results show that an internal distinction is made between vacuoles containing yeast and vacuoles containing plastic beads, and they are consistent with the hypothesis that the presence within the vacuoles of material capable of being digested prevents exocytosis.