EPITHELIAL-MESENCHYMAL INTERACTIONS IN PROSTATIC DEVELOPMENT .1. MORPHOLOGICAL OBSERVATIONS OF PROSTATIC INDUCTION BY UROGENITAL SINUS MESENCHYME IN EPITHELIUM OF THE ADULT RODENT URINARY-BLADDER
EPITHELIAL-MESENCHYMAL INTERACTIONS IN PROSTATIC DEVELOPMENT .1. MORPHOLOGICAL OBSERVATIONS OF PROSTATIC INDUCTION BY UROGENITAL SINUS MESENCHYME IN EPITHELIUM OF THE ADULT RODENT URINARY-BLADDER
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DOI：
10.1083/jcb.96.6.1662
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发表时间：
1983-01-01
影响因子：
7.8
通讯作者：
REESE, BA
中科院分区：
文献类型：
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作者：
CUNHA, GR;FUJII, H;REESE, BA
将成年大鼠和小鼠的胚胎泌尿生殖窦间充质（UGM）与膀胱上皮（尿路上皮，BLE）的组织重组体在雄性同基因宿主中培养3 - 30天。短期体内生长表明，前列腺形态发生始于成年尿路上皮基底侧的局灶性增生。实心的上皮芽伸长、分支，随后形成管道，形成前列腺腺泡。在雄性宿主中生长30天后，前列腺腺泡表现出分泌活性。UGM诱导的尿路上皮形态的显著变化伴随着指示分泌功能的精细结构特征（粗面内质网、高尔基体和分泌颗粒）的表达。在此过程中，尿路上皮细胞表达前列腺组织化学标志物（碱性磷酸酶、非特异性酯酶、糖胺聚糖）和前列腺特异性抗原。BLE内前列腺特征的表达与尿路上皮特征的丧失有关。显然，成年尿路上皮细胞对胚胎间充质诱导物保持反应性。间充质诱导的尿路上皮细胞分化变化似乎与功能活性的变化相关。
Tissue recombinants of embryonic urogenital sinus mesenchyme (UGM) and epithelium of the urinary bladder (urothelium, BLE) of adult rats and mice were grown for 3-30 d [days] in male syngeneic hosts. Short-term in vivo growth indicated that prostatic morphogenesis is initiated as focal outgrowths from the basal aspect of the adult urothelium. The solid epithelial buds elongate, branch, and subsequently canalize, forming prostatic acini. After 30 d of growth in the male hosts, prostatic acini exhibit secretory activity. The marked changes in urothelial morphology induced by the UGM are accompanied by the expression of fine-structural features indicative of secretory function (rough endoplasmic reticulum, Golgi apparatus and secretory granules). During this process, urothelial cells express prostatic histochemical markers (alkaline phosphatase, nonspecific esterase, glycosaminoglycans) and prostate-specific antigens. The expression within BLE of prostatic characteristics is associated with the loss of urothelial characteristics. Evidently adult urothelial cells retain a responsiveness to embryonic mesenchymal inductors. Mesenchyme-induced changes in urothelial cytodifferentiation appear to be coupled to changes in functional activity.