Gene expression of differentiation-specific keratins (K4, K13, K1 and K10) in oral non-dysplastic keratoses and lichen planus
Gene expression of differentiation-specific keratins (K4, K13, K1 and K10) in oral non-dysplastic keratoses and lichen planus
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DOI：
10.1034/j.1600-0714.2000.290803.x
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发表时间：
2000-09-01
影响因子：
3.3
通讯作者：
Morgan, PR
中科院分区：
文献类型：
--
作者：
Bloor, BK;Seddon, SV;Morgan, PR
通过比较原位杂交（ISH）和免疫组化（IHC）分析口腔非发育不良角化病、口腔扁平苔藓（OLP）和苔藓样反应（LR）中分化特异性角蛋白（K4、K13、K1和K10）的基因表达，以研究从非角化到帕拉角化或正角化的分化模式改变的分子变化。在蛋白质水平上，K4和K13检测均匀在基底上室的角化不全上皮，但表现出减少表达orthokeratoses，特别是在淋巴细胞的存在下。相应的成绩单仅限于基底和较低的棘细胞。K1和K10的合成在角膜上皮中上调且更明显。该研究表明，分化特异性角蛋白的模式发生了改变，尽管OLP和LR中淋巴细胞浸润的参与导致了进一步的基因调节。在这两种疾病中，K1和K10表现出转录控制，蛋白质与其转录物具有相同的分布。这代表了正常颊上皮中转录后调控的变化，其中K1和K10的mRNA比其蛋白质更广泛地表达。因此，角蛋白基因表达的模式可以响应于摩擦/吸烟刺激或免疫介导的机制而改变。
Gene expression for the differentiation-specific keratins (K4, K13, K1 and K10) was analyzed in oral non-dysplastic keratoses, oral lichen planus (OLP) and lichenoid reactions (LR) by comparative in situ hybridization (ISH) and immunohistochemistry (IHC) to investigate molecular changes in the altered differentiation pattern from non- to para- or orthokeratinization. At the protein level, K4 and K13 were detected homogeneously in the suprabasal compartment of parakeratotic epithelium but showed reduced expression in orthokeratoses, particularly in the presence of lymphocytes. Corresponding transcripts were restricted to basal and lower prickle cells. Synthesis of K1 and K10 was upregulated and more pronounced in orthokeratotic epithelia. The study showed an alteration in the pattern of differentiation-specific keratins, although involvement of the lymphocytic infiltrate in OLP and LR resulted in further gene modulation. In both diseases, K1 and K10 showed transcriptional control, proteins having the same distribution as their transcripts. This represented a change from post-transcriptional regulation in normal buccal epithelium, in which mRNAs for K1 and K10 are more widely expressed than their proteins. Thus, the pattern of keratin gene expression may be altered in response to frictional/smoking stimuli or immune-mediated mechanisms.