Spectroscopic assessment of alterations in macromolecule and small-molecule metabolites in human brain after stroke
Spectroscopic assessment of alterations in macromolecule and small-molecule metabolites in human brain after stroke
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DOI：
10.1161/hs1201.099414
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发表时间：
2001-12-01
期刊：
影响因子：
8.3
通讯作者：
Prichard, JW
中科院分区：
文献类型：
--
作者：
Graham, GD;Hwang, JH;Prichard, JW
背景和目的-我们试图测量损伤大分子和小分子（乳酸盐，N-乙酰化合物）的时间演变和空间分布。肌酸和胆碱）在中风患者中通过使用短回波时间在体内质子MR spectroscopic.Methods-Single-voxel频谱TE = 22毫秒，获得和没有反转恢复抑制的小分子共振从30检查的24名患者中风后3至214天。从未抑制的光谱中减去抑制的光谱，得到代谢物光谱，与大分子无重叠。二维光谱图像采集与大分子和小分子抑制从5额外patients. Results-Macromolecular信号升高病变相对于正常大脑，并趋于增加在亚急性期，即使乳酸峰下降。乳酸增加，在1.3 ppm的大分子信号增加，并减少N-乙酰基化合物的区域密切相关的2D spectroscopic images. Conclusions-短回波时间光谱可以在体内采集的方式，提高了信号噪声比长回波实验和解决重叠的大分子和小分子信号。在亚急性期观察到的与乳酸持续升高相关的显著大分子信号可能代表巨噬细胞或其他细胞中的移动的脂质。
Background and Purpose-We sought to measure the temporal evolution and spatial distribution of lesion macromolecules and small molecules (lactate, N-acetyl compounds. creatine, and choline) in stroke patients by using short echo time in vivo proton MR spectroscopy.Methods-Single-voxel spectra with TE = 22 ms were obtained with and without inversion recovery suppression of small-molecule resonances from 30 examinations of 24 patients 3 to 214 days after stroke. Subtraction of the suppressed from the unsuppressed spectra yielded metabolite spectra without overlap from macromolecules. Two-dimensional spectroscopic images were acquired with macromolecule and small-molecule suppression from 5 additional patients.Results-Macromolecule signals were elevated in lesions relative to normal brain and tended to increase in the subacute period, even as lactate peaks declined. Regions of increased lactate, increased macromolecule signal at 1.3 ppm, and decreased N-acetyl compounds were closely correlated in the 2D spectroscopic images.Conclusions-Short echo time spectra can be acquired in vivo in a manner that improves signal-to-noise ratio over long echo experiments and resolves overlapping macromolecule and small-molecule signals. The prominent macromolecule signals seen in the subacute period in association with persistently elevated lactate may represent mobile lipids in macrophages or other cells.