Universal model for intracellular ice formation and its growth
Universal model for intracellular ice formation and its growth
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细胞内冰形成及其生长的通用模型
DOI：
10.1002/aic.10851
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发表时间：
2006-07
期刊：
影响因子：
3.7
通讯作者：
Zhao, G
中科院分区：
文献类型：
--
作者：
Gao, DY;Luo, DW;Zhao, G
将细胞内冰体积 (IIV) 项 Vice(c,T) 引入 P. Mazur 开发的经典水传输模型中，以便修改后的模型即使在细胞内冰形成 (IIF) 后也能够预测细胞体积变化。在J. O. M. Karlsson 1994年的工作的基础上，通过耦合修正的P. Mazur模型、冰成核和扩散限制冰生长理论，描述IIF和细胞内冰（IIG）生长的新模型被开发出来。新模型可用于预测IIF、IIG、细胞内冰的最终体积分数以及细胞内冰晶的尺寸分布。新模型的主要优点是它可以为高冷冻保护剂（CPA）和低CPA浓度提供合理的细胞内信息，而无需“Avrami校正”。然后使用新模型研究冷却速率和初始 CPA 浓度对 IIF 和 IIG 的影响。提出了“盐离子（Na+和Cl−）的水合”、“甲壳玻璃化”和“局部玻璃化”三个有趣的现象，并详细研究了它们对IIF和IIG的影响。研究发现，所有这三个因素对细胞内溶液玻璃化的预测临界冷却速率都有显着影响。 © 2006 美国化学工程师学会 AIChE J，2006
The intracellular ice volume (IIV) term, Vice(c,T), is introduced into the classical water transport model developed by P. Mazur so that the modified model is capable of predicting cell volumetric change even after intracellular ice formation (IIF). By coupling the modified P. Mazur's model, ice nucleation, and the diffusion-limited ice growth theory, the new model describing IIF and the growth of the intracellular ice (IIG) is developed based on J. O. M. Karlsson's work in 1994. The new model could be used to predict IIF, IIG, the final volumetric fraction of the intracellular ice, and the size distribution of the intracellular ice crystals. The major advantage of the new model is that it can provide reasonable intracellular information for both high cryoprotective agent (CPA) and low CPA concentrations without “Avrami correction”. The new model is then used to study the effect of cooling rate and initial CPA concentration on IIF and IIG. Three interesting phenomena, “hydration of salt ions (Na+ and Cl−)”, “crustaceous vitrification”, and “local vitrification” are presented, and their effects on IIF and IIG are studied in detail. It is found that all three of these factors have significant influences on the predicted critical cooling rate for the vitrification of the intracellular solution. © 2006 American Institute of Chemical Engineers AIChE J, 2006
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影响因子：
2.7
作者：
S. Aggarwal;K. Diller;C. Baxter
通讯作者：
S. Aggarwal;K. Diller;C. Baxter
影响因子：
3.4
作者：
Irimia, D;Karlsson, JOM
通讯作者：
Karlsson, JOM
影响因子：
2.7
作者：
M. Wusteman;M. Robinson;D. Pegg
通讯作者：
M. Wusteman;M. Robinson;D. Pegg
影响因子：
3.4
作者：
KARLSSON, JOM;CRAVALHO, EG;TONER, M
通讯作者：
TONER, M
影响因子：
2.7
作者：
J. Karlsson
通讯作者：
J. Karlsson