Characterization of nuclear PTEN and its post translational modifications
Characterization of nuclear PTEN and its post translational modifications
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DOI：
10.1016/j.ymeth.2015.01.006
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发表时间：
2015-05-01
期刊：
影响因子：
4.8
通讯作者：
Stambolic, V.
中科院分区：
文献类型：
--
作者：
Ho, J.;Bassi, C.;Stambolic, V.
PTEN 体细胞功能丧失突变存在于多种人类恶性肿瘤中。我们最近的工作表明 PTEN 的核功能与基因组完整性的维持有关。基因毒性应激后 PTEN 的正确亚细胞定位是通过细胞机制协调的，该机制涉及 SUMO 化和 ATM 介导的磷酸化的翻译后修饰。在这里，我们总结了可用于表征 DNA 损伤背景下核 PTEN 的 SUMO 化和磷酸化状态的生化和基于细胞的方法。此外，我们还描述了确定 SUMO-PTEN 在同源重组 DNA 修复中的生物学功能的测定方法。这些方法将有助于阐明 PTEN 在维持基因组稳定性中作用的精确分子机制。 (C) 2015 Elsevier Inc. 保留所有权利。
Somatic loss-of-function mutations of PTEN are found in a variety of human malignancies. Our recent work demonstrated that the nuclear function of PTEN is implicated in the maintenance of genome integrity. Proper subcellular localization of PTEN following genotoxic stress is coordinated by a cellular mechanism that involves post-translational modification by SUMOylation and ATM-mediated phosphorylation. Here we summarize biochemical and cell-based methodologies that can be used to characterize the SUMOylation and phosphorylation state of nuclear PTEN in the context of DNA damage. In addition, we describe assays to determine the biological function of SUMO-PTEN in homologous recombination DNA repair. These methods will help elucidate the precise molecular mechanisms of PTEN's role in the maintenance of genomic stability. (C) 2015 Elsevier Inc. All rights reserved.