Differential expression of CD8 epitopes amongst porcine CD8‐positive functional lymphocyte subsets
Differential expression of CD8 epitopes amongst porcine CD8‐positive functional lymphocyte subsets
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DOI：
10.1046/j.1365-2567.1997.00308.x
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发表时间：
1997-09
期刊：
影响因子：
6.4
通讯作者：
H. Yang;R. Parkhouse
中科院分区：
文献类型：
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作者：
H. Yang;R. Parkhouse
猪是免疫生物学研究的有用模型，因为它有一个高度异质的淋巴细胞池，包含几个在人类和啮齿动物中不易获得的亚群。特别是，CD8+细胞含有多种淋巴细胞亚群，如αβ-T细胞、γδ-T细胞、CD4CD8双阳性(DP)细胞和自然杀伤(NK)细胞。为了进一步确定这些亚群，我们选择了四种对CD8+细胞具有不同反应性的单抗。因此，单抗CD8.1(PPT20)与CD8hi和CD8lo亚群的结合方式与传统的抗CD8类似。CD8.2(PPT21)单抗虽然能与所有CD8+细胞结合，但与CD8hi反应较好。另外两株单抗CD8hiαβ-T细胞亚群具有特异性。这些结果与免疫沉淀、共调节和酶联免疫吸附实验相结合，表明CD8.1和CD8.2CD8与α链反应，CD8.3CD8.4与CD8β链反应。组织分布研究表明，CD_8~+胸腺细胞和外周血CD8~+αβ-T细胞同时表达CD_8~+α链和β链，而CD_4~+CD_8+αβ-T细胞、CD_810~+γδ-T细胞和NK细胞仅表达CD_8~α链。功能研究表明，CD8hiαβ-T细胞和CD8loγδ-T细胞是CD3重定向细胞毒作用的效应细胞。
The swine is a useful model for immunobiological studies as it has a highly heterogeneous lymphocyte pool, containing several subsets not easily accessible in humans and rodents. In particular, the CD8‐positive (CD8+) cells contain a variety of lymphocyte subsets, such as αβ‐T cells, γδ‐T cells, CD4 CD8 double‐positive (DP) cells and natural killer (NK) cells. In order to define these subsets further, we have selected four monoclonal antibodies (mAb) with differential reactivity on CD8+ cells. Thus, mAb CD8.1 (PPT20) bound to CD8hi and CD8lo subpopulations in a similar way to the conventional anti‐CD8. The mAb CD8.2 (PPT21), though binding to all of the CD8+ cells, reacted preferably with CD8hi. Two other mAb, CD8.3 (PPT22) and CD8.4 (PPT23), were specific for CD8hiαβ‐T‐cell subpopulation. These results, complemented by immunoprecipitation, co‐modulation and enzyme‐linked immunosorbent assay experiments, suggest that CD8.1 and CD8.2 react putatively with the CD8α‐chain and CD8.3 and CD8.4 with the CD8β‐chain. Tissue distribution studies revealed that CD8+ thymocytes and peripheral CD8hiαβ‐T cells expressed both putative CD8α‐ and β‐chains while peripheral CD4+CD8+αβ‐T cells, CD8loγδ‐T cells and NK cells expressed only putative CD8α‐chain. Functional studies indicated that the CD8hiαβ‐T and CD8loγδ‐T cells were effector cells in the CD3‐redirected cytotoxicity.