nlz gene family is required for hindbrain patterning in the zebrafish
nlz gene family is required for hindbrain patterning in the zebrafish
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DOI：
10.1002/dvdy.20001
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发表时间：
2004-04-01
影响因子：
2.5
通讯作者：
Sive, H
中科院分区：
文献类型：
--
作者：
Hoyle, J;Tang, YP;Sive, H
这项研究描述了保守的NIZ基因家族，其成员编码异常的锌指蛋白。在斑马鱼神经抑制剂中，NIZ1和新分离的Niz2均在假定后脑和中脑/后脑边界中表达，其中Niz1的表达取决于PAX2A。此外，在假定的中脑和脑脑中，NIZ2在前方更向前表达。胃阶段中NIZ蛋白的过表达抑制后脑发育。特别是，异位表达的Niz1抑制了未来菱形2和3（R2，R3）的形成，而相邻的R1和R4则不受影响。相反，通过表达反义形态固定的寡聚物，同时还原Niz1和Niz2蛋白功能会导致未来R3和R5的扩展，并与R4相关的损失。这些数据表明，NIZ基因家族的一个功能是指定或维持R4身份，并限制在后脑形成期间R3和R5。 （c）2004 Wiley-Liss，Inc。
This study describes the conserved niz gene family whose members encode unusual zinc finger proteins. In the zebrafish neurectoderm, both nIz1 and the newly isolated nIz2 are expressed in the presumptive hindbrain and midbrain/hindbrain boundary, where expression of nIz1 is dependent on pax2a. In addition, nIz2 is uniquely expressed more anteriorly, in the presumptive midbrain and diencephalon. Overexpression of NIz proteins during gastrula stages inhibits hindbrain development. In particular, ectopically expressed NIz1 inhibits formation of future rhombomeres 2 and 3 (r2, r3), whereas neighboring r1 and r4 are not affected. Conversely, simultaneous reduction of NIz1 and NIz2 protein function by expression of antisense morpholino-modified oligomers leads to expansion of future r3 and r5, with associated loss of r4. These data indicate that one function of the niz gene family is to specify or maintain r4 identity, and to limit r3 and r5 during hindbrain formation. (C) 2004 Wiley-Liss, Inc.