Salt stress-induced chloroplastic hydrogen peroxide stimulates pdTPI sulfenylation and methylglyoxal accumulation
Salt stress-induced chloroplastic hydrogen peroxide stimulates pdTPI sulfenylation and methylglyoxal accumulation
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DOI：
10.1093/plcell/koad019
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发表时间：
2023-01-25
期刊：
影响因子：
11.6
通讯作者：
Lu，Ying-Tang
中科院分区：
文献类型：
--
作者：
Fu，Zheng-Wei;Feng，Yu-Rui;Lu，Ying-Tang
高盐度是一种影响全球约20%灌溉耕地的不利环境因素，通过引起氧化应激、破坏细胞成分和干扰全球代谢来抑制植物生长和发育。然而，活性氧是否以及如何干扰盐胁迫植物的代谢仍然是一个谜。在这里，我们报告说，盐诱导的过氧化氢（H2O2）抑制质体磷酸丙糖异构酶（pdTPI）的活性，以促进甲基乙二醛（MG）的积累，并刺激半胱氨酸74处的pdTPI的亚磺酰化。我们还表明，MG是限制植物生长的关键因素，因为MG水平的降低完全挽救了pdt突变体的生长迟缓和抑制的盐胁迫耐受性。此外，靶向过氧化氢酶2进入叶绿体，以防止盐诱导的过氧化氢积累赋予盐胁迫的耐受性，揭示了叶绿体过氧化氢在盐引起的植物损害的作用。此外，我们证明了H2O2介导的MG积累反过来诱导H2O2的产生，从而形成一个调节回路，进一步抑制盐胁迫植物中的pdTPI活性。因此，我们的研究结果，说明盐胁迫诱导MG生产抑制植物生长。
High salinity, an adverse environmental factor affecting about 20% of irrigated arable land worldwide, inhibits plant growth and development by causing oxidative stress, damaging cellular components, and disturbing global metabolism. However, whether and how reactive oxygen species disturb the metabolism of salt-stressed plants remain elusive. Here, we report that salt-induced hydrogen peroxide (H2O2) inhibits the activity of plastid triose phosphate isomerase (pdTPI) to promote methylglyoxal (MG) accumulation and stimulates the sulfenylation of pdTPI at cysteine 74. We also show that MG is a key factor limiting the plant growth, as a decrease in MG levels completely rescued the stunted growth and repressed salt stress tolerance of thepdtpimutant. Furthermore, targeting CATALASE 2 into chloroplasts to prevent salt-induced overaccumulation of H2O2conferred salt stress tolerance, revealing a role for chloroplastic H2O2in salt-caused plant damage. In addition, we demonstrate that the H2O2-mediated accumulation of MG in turn induces H2O2production, thus forming a regulatory loop that further inhibits the pdTPI activity in salt-stressed plants. Our findings, therefore, illustrate how salt stress induces MG production to inhibit the plant growth.