Enhancement of lignan biosynthesis in suspension cultures of Linum nodiflorum by coronalon, indanoyl-isoleucine and methyl jasmonate
Enhancement of lignan biosynthesis in suspension cultures of Linum nodiflorum by coronalon, indanoyl-isoleucine and methyl jasmonate
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DOI：
10.1007/s00425-005-0019-9
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发表时间：
2005-11-01
期刊：
影响因子：
4.3
通讯作者：
Petersen, M
中科院分区：
文献类型：
--
作者：
Berim, A;Spring, O;Petersen, M
研究了人工合成的诱导子冠醚（coronalon）和茚满酰异亮氨酸（indanoyl-isoleucine）以及茉莉酸甲酯（MeJA）对亚麻悬浮培养细胞木脂素积累和生物合成的影响。6-甲氧基鬼臼毒素（MPTOX）的产量可提高10倍以上，最高可达细胞干重的2.5%以上。通过在培养期的第四天施用50 μ M的合成诱导子并在第十天提取产物获得最高产量。一个额外的木脂素积累在激发子处理的文化。其结构通过广泛的1D和2D NMR测量来阐明，揭示其身份为5 '-去甲氧基-MPTOX（5'-dMPTOX）。其平均含量高达细胞干重的5%以上。诱导剂的加入对生长只有轻微的影响。茉莉酸甲酯对L.结果表明，在nodiflorum细胞中，MPTOX和5 '-dMPTOX含量分别高达细胞干重的1.4%和2.1%。脱氧鬼臼毒素6-羟化酶和β-peltatin 6-O-甲基转移酶的活动，参与MPTOX生物合成的两种酶，分别增加到21.9倍和14.6倍，在处理的文化。
The effect of the two synthetic elicitors coronalon and indanoyl-isoleucine and of methyl jasmonate (MeJA) on the accumulation and biosynthesis of lignans by cell suspension cultures of Linum nodiflorum (Linaceae) was investigated. The production of 6-methoxypodophyllotoxin (MPTOX) could be increased more than tenfold, the maximal content reaching up to over 2.5% of the cell dry weight. The highest yield was achieved by administering 50 mu M of the synthetic elicitors on the fourth day and extracting the products on the tenth day of the culture period. An additional lignan accumulated in elicitor-treated cultures. Its structure was elucidated by extensive 1D and 2D NMR measurements, revealing its identity as 5'-demethoxy-MPTOX (5'-dMPTOX). Its average content amounted up to over 5% of the cell dry weight. Growth was only slightly affected by the addition of the elicitors. Methyl jasmonate exerted a moderate stimulating effect on the L. nodiflorum cells with MPTOX and 5'-dMPTOX contents going up to 1.4 and 2.1% of the cell dry weight, respectively. The activities of deoxypodophyllotoxin 6-hydroxylase and beta-peltatin 6-O-methyltransferase, two enzymes involved in MPTOX biosynthesis, were increased up to 21.9-fold and 14.6-fold, respectively, in the treated cultures.