Expression analysis of peptidergic enteroendocrine cells in the silkworm Bombyx mori
Expression analysis of peptidergic enteroendocrine cells in the silkworm Bombyx mori
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DOI：
10.1007/s00441-022-03666-1
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发表时间：
2022-07-13
影响因子：
3.6
通讯作者：
Zitnan，Dusan
中科院分区：
文献类型：
--
作者：
Roller，Ladislav;Daubnerova，Ivana;Zitnan，Dusan
昆虫中肠的肠内分泌细胞（enteroendocrine cells，ECs）通过释放多种多肽来调节食物摄入、胃肠活动和全身代谢。在这里，我们进行了一个全面的映射EC产生不同的调节肽的幼虫中肠的家蚕。总之，我们确定了20肽基因表达在不同的EC中的特定区域的中肠。转录特异性原位杂交结合抗体染色揭示了大约30个EC亚群，每个亚群产生一种独特的肽或几种不同肽的组合。这种多样性的功能意义和不同肠内分泌肽的具体作用在很大程度上是未知的。本研究的结果突出了中肠作为昆虫调节分子的主要内分泌/旁分泌来源的重要性，并为阐明EC在幼虫摄食和发育过程中的功能提供了重要信息。
Enteroendocrine cells (ECs) in the insect midgut respond to physiological changes in the intestine by releasing multiple peptides to control food intake, gastrointestinal activity and systemic metabolism. Here, we performed a comprehensive mapping of ECs producing different regulatory peptides in the larval midgut ofBombyx mori. In total, we identified 20 peptide genes expressed in different ECs in specific regions of the midgut. Transcript-specific in situ hybridisation combined with antibody staining revealed approximately 30 subsets of ECs, each producing a unique peptide or a combination of several different peptides. Functional significance of this diversity and specific roles of different enteroendocrine peptides are largely unknown. Results of this study highlight the importance of the midgut as a major endocrine/paracrine source of regulatory molecules in insects and provide important information to clarify functions of ECs during larval feeding and development.