Hyphae of a vesicular—arbuscular mycorrhizal fungus maintain infectivity in dry soil, except when the soil is disturbed
Hyphae of a vesicular—arbuscular mycorrhizal fungus maintain infectivity in dry soil, except when the soil is disturbed
复制标题
DOI：
10.1111/j.1469-8137.1989.tb00314.x
复制
发表时间：
1989-05
期刊：
影响因子：
9.4
通讯作者：
D. Jasper;L. Abbott;A. Robson
中科院分区：
文献类型：
--
作者：
D. Jasper;L. Abbott;A. Robson
摘要 我们检验了这样的假设：干燥土壤中 VA 菌根真菌的菌丝仍然具有感染性，但如果土壤受到干扰，感染性就会降低。我们使用分体盆和密封的尼龙网圆筒来制作大量干燥的土壤，其中含有无尾孢菌菌丝。和特拉普（Trappe），其附着在网外，附着在金合欢植物的根部。 （拉比尔）H.温德尔。土壤原位干燥后，菌丝从根部脱离而未扰动土壤，或扰动含有菌丝的土壤。然后将这些处理后菌丝的感染性与完整菌丝的感染性进行比较。在基质势达到-21 MPa的土壤中，滑鼠的外部菌丝仍然具有高度感染性。从原始寄主植物根部脱离的菌丝同样具有感染性。然而，含有外部菌丝的干燥土壤的扰动严重降低了菌丝的感染性。荧光染色后，具有荧光核的真菌结构在不具有荧光核的A. Saligna的根内和附着在根部上均容易可见。
SUMMARY We examined the hypothesis that hyphae of a VA mycorrhizal fungus in dry soil remain infective, but that the infectivity decreases if the soil is disturbed. We used split-pots, and sealed cylinders of nylon mesh, to create volumes of dry soil which contained hyphae of Acaulospora laevis Gerd. and Trappe that were attached, outside the mesh, to roots of living plants of Acacia satigna. (Labill.) H. Wendl. After the soil was dried in situ, the hyphae were detached from the roots without soil disturbance, or the soil containing the hyphae was disturbed. The infectivity of the hyphae after these treatments was then compared with that of intact hyphae. External hyphae of A. laevis remained highly infective in soil in which the matric potential had reached −21 MPa. Hyphae detached from the original host plant roots were equally infective. However, disturbance of the dry soil containing the external hyphae severely reduced the infectivity of the hyphae. After fluorescent staining, fungal structures with fluorescing nuclei were readily visible both within, and attached to, roots of A. Saligna which had no fluorescing nuclei.