Complement-mediated antibody-dependent enhancement of HIV-1 infection requires CD4 and complement receptors.
Complement-mediated antibody-dependent enhancement of HIV-1 infection requires CD4 and complement receptors.
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HIV-1 感染的补体介导的抗体依赖性增强需要 CD4 和补体受体。
DOI：
10.1016/0042-6822(90)90449-2
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发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
Mitchell,WM
中科院分区：
文献类型：
--
作者：
RobinsonJr,WE;Montefiori,DC;Mitchell,WM
本研究证明，体外 HIV-1 感染的补体介导的抗体依赖性增强 (C'-ADE) 可被针对 CD4 和补体受体 2 型 (CR2) 的鼠单克隆抗体阻断，而在缺乏 C'-ADE 的情况下，HIV-1 感染可被抗 CD4 单克隆抗体阻断，但不能被抗 CR2 单克隆抗体阻断。抗 CR2 鼠单克隆抗体 OKB7 在浓度大于 1 μg/ml 时可阻断 HIV-1 感染的 C'-ADE。抗 CD4 单克隆抗体 OKT4a（而非 OKT4f）在浓度大于 0.06 μg/ml 时阻断 C'-ADE。 HIV-1 感染通过细胞病变效应、间接免疫荧光和逆转录酶释放进行定量。从这些体外研究看来，HIV-1感染的C'-ADE需要CD4和补体受体两者，而在没有抗体和补体的情况下HIV-1感染仅需要CD4。
In this study it is demonstrated that complement-mediated antibody-dependent enhancement (C'-ADE) of HIV-1 infectionin vitrois blocked by murine monoclonal antibodies to CD4 and complement receptor type 2 (CR2) while HIV-1 infection in the absence of C'-ADE is blocked by anti-CD4 but not anti-CR2 monoclonal antibodies. The anti-CR2 murine monoclonal antibody, OKB7, blocked C'-ADE of HIV-1 infection at concentrations greater than 1 μg/ml. The anti-CD4 monoclonal antibody, OKT4a, but not OKT4f blocked C'-ADE at concentrations greater than 0.06 μg/ml. HIV-1 infections were quantitated by cytopathic effect, indirect immunofluorescence, and reverse transcriptase release. It appears from thesein vitrostudies that C'-ADE of HIV-1 infection requires both CD4 and complement receptors while HIV-1 infection in the absence of antibody and complement requires only CD4.