Determination of hemoglobin derivatives with IL 282 CO-oximeter as compared with a manual spectrophotometric five-wavelength method.
Determination of hemoglobin derivatives with IL 282 CO-oximeter as compared with a manual spectrophotometric five-wavelength method.
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使用 IL 282 CO 血氧计测定血红蛋白衍生物，并与手动分光光度五波长法进行比较。
DOI：
10.1093/clinchem/27.11.1903
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发表时间：
1981
影响因子：
9.3
通讯作者：
W. Zijlstra
中科院分区：
文献类型：
--
作者：
A. Zwart;A. Buursma;B. Oeseburg;W. Zijlstra
用IL 282CO血氧仪测定的血红蛋白衍生物与人工五波长法测定的结果有很好的相关性，而手动五波长法又与一种或两种衍生物的既定方法进行了对照。两种方法对总血红蛋白的测量结果几乎相同。至于氧合血红蛋白、碳氧合血红蛋白和半球蛋白，两种方法之间的一致性是公平的。虽然IL-282一氧化碳血氧仪尚未被建造用于硫代血红蛋白的测定，但似乎该仪器仍能给出该血红蛋白衍生物的存在的强烈指示。胎儿血液IL-282的结果应该谨慎使用，特别是因为可能会出现虚假高的HbCO读数。
Hemoglobin derivatives as determined with the IL 282 CO-Oximeter correlated well with results by a manual five-wavelength method, which in turn had been checked against established methods for one or two derivatives. Measurement of total hemoglobin yielded almost identical results with both methods. As for oxyhemoglobin, carboxyhemoglobin, and hemiglobin, agreement between the two methods was fair. Although the IL 282 CO-Oximeter has not been constructed for the determination of sulfhemoglobin, it appeared that the instrument can still give a strong indication as to the presence of this hemoglobin derivative. Results from the IL 282 for fetal human blood should be used with caution, especially because of the possibility of falsely high HbCO readings.