LATENT HUMAN HERPESVIRUS-6 INFECTION OF HUMAN MONOCYTES MACROPHAGES
LATENT HUMAN HERPESVIRUS-6 INFECTION OF HUMAN MONOCYTES MACROPHAGES
复制标题
DOI：
10.1099/0022-1317-72-6-1401
复制
发表时间：
1991-06-01
影响因子：
3.8
通讯作者：
YAMANISHI, K
中科院分区：
文献类型：
--
作者：
KONDO, K;KONDO, T;YAMANISHI, K
应用聚合酶链反应（PCR）技术检测了急性期和恢复期急性期 虽然在急性期的非贴壁和贴壁单核细胞中可以检测到DNA，但在恢复期主要在贴壁细胞中检测到;此外，在健康成人的贴壁细胞中发现了病毒DNA。 当贴壁单个核细胞体外培养时，发现病毒在感染前体外培养7天的分化细胞中复制良好。 当细胞培养超过1个月时，未观察到可检测的抗原和病毒生长的证据，但可检测到病毒DNA。 用佛波醇酯处理这些明显潜伏感染的单核细胞，之后可以从培养物中回收病毒。 因此，我们已经开发了一个体外潜伏系统的HHV-6，我们的研究结果表明，HHV-6可以潜伏感染单核细胞在体内和体外，它可能会被重新激活的细胞中的一些因素。
Human herpesvirus 6 (HHV-6) DNA was detected in peripheral blood from exanthem subitum patients during the acute and convalescent phases of infection using the polymerase chain reaction. Although DNA could be detected in non-adherent and adherent mononuclear cells during the acute phase, it was detected predominantly in adherent cells during the convalescent phase; furthermore, viral DNA was found in adherent cells of healthy adults. When adherent mononuclear cells were cultured in vitro, virus was found to replicate well in differentiated cells cultured for 7 days in vitro before infection. When cells were cultured for more than 1 month, no detectable antigen and no evidence of virus growth was observed, but viral DNA could be detected. These apparently latently infected monocytes were treated with phorbol ester, after which virus could be recovered from the cultures. Therefore, we have developed an in vitro latency system for HHV-6; our results suggest that HHV-6 may latently infect monocytes in vivo and in vitro and that it may be reactivated in cells by some factors.