Development and Application of SSR Markers Related to Genes Involved in Leaf Adaxial-Abaxial Polarity Establishment in Chinese Cabbage (Brassica rapaL. ssp.pekinensis)
Development and Application of SSR Markers Related to Genes Involved in Leaf Adaxial-Abaxial Polarity Establishment in Chinese Cabbage (Brassica rapaL. ssp.pekinensis)
复制标题
大白菜叶近轴极性建立相关基因SSR标记的开发及应用
DOI：
10.3389/fgene.2020.00773
复制
发表时间：
2020-07-23
影响因子：
3.7
通讯作者：
Shen, Shuxing
中科院分区：
文献类型：
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作者：
Gao, Ying;Lu, Yin;Shen, Shuxing
白菜（Brassica rapaL.）北京亚种），叶的近轴-远轴（ad-ab）极性与叶的弯曲度密切相关，这是形成叶球的一个重要因素。因此，鉴定叶片ad-ab极性相关基因并研究其遗传变异可能有助于阐明叶球形成的机制。通过比较拟南芥（Arabidopsis thaliana，A. thaliana）中，发现分布在10条染色体上的41个候选基因与大白菜ad-ab极性的建立有关。在大白菜中检测到10个单拷贝、14个双拷贝和1个三拷贝的直向同源基因。基因结构和保守结构域分析表明，41个候选基因的外显子数从1到25不等，大多数基因共享保守基序1，6和10。基于41个候选基因，检测到341个简单重复序列（SSR），包括5种复制类型：单，双，三，五，六核苷酸复制。利用其中的323个SSR位点设计了969对特异引物，随机选取362对引物对12份不同结球类型的大白菜材料进行了评价。以重叠结球白菜“14 Q-141”和外卷结球白菜“14 Q-279”的杂种F（2）群体214个株系为材料，利用23对扩增出清晰多态性条带的引物，结合127个标记，构建了一张连锁图。“结果表明，遗传连锁图和物理图谱上的标记序列基本一致。本研究有助于加快大白菜叶球品质育种进程。
In Chinese cabbage (Brassica rapaL. ssp.pekinensis), leaf adaxial-abaxial (ad-ab) polarity is tightly related to leaf incurvature, an essential factor for the formation of leafy heads. Therefore, identification of the genes responsible for leaf ad-ab polarity and studying their genetic variation may clarify the mechanism of leafy head formation. By comparing the sequences of the genes regulating leaf ad-ab polarity development inArabidopsis thaliana(A. thaliana), 41 candidate genes distributed on 10 chromosomes were found to be responsible for the establishment of ad-ab polarity in Chinese cabbage. Orthologous genes, including 10 single copies, 14 double copies, and one triple copies, were detected in the Chinese cabbage. The gene structure and conserved domain analyses showed that the number of exons of the 41 candidate genes range from one to 25, and that most genes share the conserved motifs 1, 6, and 10. Based on the 41 candidate genes, 341 simple sequence repeats (SSRs) were detected, including five replicated types: single, double, triple, quintuple, and sextuple nucleotide replications. Among these sequence repeat (SSR) loci, 323 loci were used to design 969 specific primers, and 362 primer pairs were selected randomly and evaluated using 12 Chinese cabbage accessions with different heading types. 23 primer pairs resulting with clear, polymorphic bands, combined with other 127 markers, was used to construct a linkage map by using an F(2)population containing 214 lines derived from the hybrid of the overlapping heading Chinese cabbage "14Q-141" and the outward curling heading Chinese cabbage "14Q-279." The result showed that the sequences of markers in the genetic linkage map and the physical map was consistent in general. Our study could help to accelerate the breeding process of leafy head quality in Chinese cabbage.