Bacterial amyloids.
Bacterial amyloids.
复制标题
细菌淀粉样蛋白。
DOI：
10.1007/978-1-61779-551-0_21
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Chapman,MatthewR
中科院分区：
文献类型：
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作者：
Zhou,Yizhou;Blanco,LuzP;Smith,DanielR;Chapman,MatthewR
许多细菌可以在其细胞表面组装功能性淀粉样蛋白纤维。大多数细菌淀粉样蛋白有助于生物膜或其他群落行为，其中细胞与表面或另一细胞相互作用。与所有功能性淀粉样蛋白一样，细菌淀粉样蛋白与疾病相关的真核淀粉样蛋白具有相同的结构和生化特性。淀粉样蛋白结合淀粉样蛋白特异性染料（如刚果红）的一般能力及其对变性的抗性为评分和定量细菌淀粉样蛋白形成提供了有用的工具。在这里，我们提出了研究细菌淀粉样蛋白的基本方法，重点是研究肠杆菌科表达的卷曲淀粉样蛋白纤维。这些方法利用了淀粉样蛋白的特定着色和生物物理特性。这里描述的方法很简单，可以很容易地应用于任何现代分子生物学实验室研究其他细菌淀粉样蛋白。
Many bacteria can assemble functional amyloid fibers on their cell surface. The majority of bacterial amyloids contribute to biofilm or other community behaviors where cells interact with a surface or with another cell. Bacterial amyloids, like all functional amyloids, share structural and biochemical properties with disease-associated eukaryotic amyloids. The general ability of amyloids to bind amyloid-specific dyes, such as Congo red, and their resistance to denaturation have provided useful tools for scoring and quantifying bacterial amyloid formation. Here, we present basic approaches to study bacterial amyloids by focusing on the well-studied curli amyloid fibers expressed byEnterobacteriaceae. These methods exploit the specific tinctorial and biophysical properties of amyloids. The methods described here are straightforward and can be easily applied by any modern molecular biology lab for the study of other bacterial amyloids.