Schizosaccharomyces pombe Homologs of Human DJ-1 Are Stationary Phase-Associated Proteins That Are Involved in Autophagy and Oxidative Stress Resistance.
Schizosaccharomyces pombe Homologs of Human DJ-1 Are Stationary Phase-Associated Proteins That Are Involved in Autophagy and Oxidative Stress Resistance.
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人类 DJ-1 的裂殖酵母同源物是参与自噬和氧化应激抵抗的固定相相关蛋白。
DOI：
10.1371/journal.pone.0143888
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Su Y;Chen C;Huang L;Yan J;Huang Y
帕金森病蛋白DJ-1参与多种细胞功能，包括二羰基化合物的解毒、自噬和氧化应激反应。DJ-1同源物广泛存在于原核生物和真核生物中，构成了似乎参与应激反应的蛋白质超家族。粟酒裂殖酵母含有6个DJ-1同源物，命名为Hsp3101-Hsp3105和Sdj1（以前命名为SpDJ-1）。在这里，我们表明，这六个基因中的任何一个的删除以某种方式影响自噬在延长的稳定期。此外，这些DJ-1同源物中的每一个的缺失导致稳定期存活减少。sdj1的缺失也增加了稳定期细胞对过氧化氢（H2O2）诱导的氧化应激的敏感性，而sdj1的过表达具有相反的效果。与它们在稳定期中的作用一致，hsp3101、hsp3102、hsp3105和sdj1的表达以及hsp3103和hsp3104在较小程度上在稳定期中增加。热休克蛋白3101，3102，3105和sdj1的诱导涉及Sty1调节的转录因子Atf1，但不涉及转录因子Pap1。我们的结果坚定地建立了S。DJ-1的粟酒裂殖酵母同源物是稳定期相关蛋白，可能参与S.粟酒裂殖酵母细胞
The Parkinson′s disease protein DJ-1 is involved in various cellular functions including detoxification of dicarbonyl compounds, autophagy and oxidative stress response. DJ-1 homologs are widely found in both prokaryotes and eukaryotes, constituting a superfamily of proteins that appear to be involved in stress response. Schizosaccharomyces pombe contains six DJ-1 homologs, designated Hsp3101-Hsp3105 and Sdj1 (previously named SpDJ-1). Here we show that deletion of any one of these six genes somehow affects autophagy during prolonged stationary phase. Furthermore, deletions of each of these DJ-1 homologs result in reduced stationary phase survival. Deletion of sdj1 also increases the sensitivity of stationary-phase cells to oxidative stress induced by hydrogen peroxide (H2O2) whereas overexpression of sdj1 has the opposite effect. Consistent with their role in stationary phase, expression of hsp3101, hsp3102, hsp3105 and sdj1, and to a lesser extent hsp3103 and hsp3104, is increased in stationary phase. The induction of hsp3101, hsp3102, hsp3105 and sdj1 involves the Sty1-regulated transcription factor Atf1 but not the transcription factor Pap1. Our results firmly establish that S. pombe homologs of DJ-1 are stationary-phase associated proteins and are likely involved in autophagy and antioxidant defense in stationary phase of S. pombe cells.