IMMUNOHISTOCHEMICAL LOCALIZATION OF THYROTROPIN-RELEASING-HORMONE IN THE RAT HYPOTHALAMUS AND PITUITARY
IMMUNOHISTOCHEMICAL LOCALIZATION OF THYROTROPIN-RELEASING-HORMONE IN THE RAT HYPOTHALAMUS AND PITUITARY
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DOI：
10.1210/endo-111-1-55
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发表时间：
1982-01-01
期刊：
影响因子：
4.8
通讯作者：
JACKSON, IMD
中科院分区：
文献类型：
--
作者：
LECHAN, RM;JACKSON, IMD
用5%丙烯醛快速固定大鼠大脑后，使用过氧化物酶-抗过氧化物酶技术证实了免疫反应性TRH在大鼠下丘脑和垂体中的分布。在整个下丘脑的神经元过程中鉴定出广泛的反应产物，在正中隆起、背内侧核、室旁核的小细胞分裂、穹窿周区域、室周核和终板的血管器中具有密集的标记。在整个垂体后叶中还发现了免疫反应性 TRH 的显着积累，其中纤维似乎以葡萄状肿胀终止。过氧化物酶阳性的核周体在秋水仙碱预处理后最为明显，分布在下丘脑的许多区域。免疫反应性神经元密度最大的是视交叉上视前核、室旁核的细细胞细分、穹窿周区、背内侧核和下丘脑基底外侧核。 TRH 作为促垂体激素、垂体后叶调节剂以及下丘脑其他区域神经元的神经递质或神经调节剂的作用得到了支持。
The distribution of immunoreactive TRH in the rat hypothalamus and pituitary was demonstrated using the peroxidase-antiperoxidase technique after rapid fixation of the rat brain with 5% acrolein. Widespread reaction product was identified in neuronal processes throughout the hypothalamus, with dense labeling in the median eminence, dorsomedial nucleus, parvocellular division of the paraventricular nucleus, perifornical region, periventricular nucleus and organum vasculosum of the lamina terminalis. A striking accumulation of immunoreactive TRH was also noted throughout the posterior pituitary, where fibers appeared to terminate in grape-like swellings. Peroxidase-positive perikarya were best seen after colchicine pretreatment and were distributed in many regions of the hypothalamus. The greatest density of immunoreactive neurons was in the suprachiasmatic preoptic nucleus, parvocellular subdivision of the paraventricular nucleus, perifornical region, dorsomedial nucleus and baso-lateral hypothalamus. The role of TRH as a hypophysiotropic hormone, a regulator of the posterior pituitary and a neurotransmitter or neuromodulator of neurons in other regions of the hypothalamus is supported.