Enzymatic synthesis of a tetraglycosylceramide by a galactosyltransferase from rabbit bone marrow.
Enzymatic synthesis of a tetraglycosylceramide by a galactosyltransferase from rabbit bone marrow.
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通过兔骨髓中的半乳糖基转移酶酶促合成四糖基神经酰胺。
DOI：
10.1016/s0021-9258(19)45585-3
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
S. Basu
中科院分区：
文献类型：
--
作者：
M. Basu;S. Basu
在兔骨髓匀浆中检测到一种半乳糖转移酶，它能将半乳糖β-(N-乙酰)-氨基葡萄糖(1-→-3)-O-β-半乳糖(1-→-4)-葡萄糖(1-→-1)神经酰胺转化为半乳糖基-O-β-(N-乙酰)氨基葡萄糖(1-→-3)-O-β-半乳糖(1→4)-葡萄糖(1→1)神经酰胺。兔骨髓经Triton X-100匀浆和100,000×g离心后，上清液中有该酶的存在。对反应产物进行分离、提纯，并对其成分进行分析。它与抗XIV肺炎球菌多糖马血清形成沉淀线，并与兔红细胞B活性五糖神经酰胺的四糖神经酰胺形成沉淀线。UDP-半乳糖不能被半乳糖或半乳糖-1-P取代，甘氨酸神经酰胺和UDP-半乳糖的Km值分别为5.0×10~(-4)M和1.2×10~(-4)M。
A galactosyltransferase that transferred galactose from UDP-galactose toO-β-(N-acetyl)-glucosaminosyl (1 → 3)-O-β-galactosyl (1 → 4) glucosyl (1 → 1) ceramide to give galactosyl-O-β-(N-acetyl)glucosaminosyl (1 → 3)-O-β-galactosyl (1 → 4)-glucosyl (1 → 1) ceramide was detected in homogenates of rabbit bone marrow. The enzyme was present in the supernatant solution obtained after homogenization of rabbit bone marrow in the presence of Triton X-100 and centrifugation at 100,000xg. The product of the reaction was isolated, purified, and analyzed for its constituents. It formed a precipitin line with anti-type XIV pneumococcal polysaccharide horse serum, and the line fused with that of tetraglycosylceramide derived from rabbit erythrocyte B active pentaglycosylceramide. UDP-galactose could not be replaced by galactose or galactose-1-P; theKmvalues were 5.0x10-4mand 1.2x10-4mfor the triglycosylceramide and UDP-galactose, respectively.